antibody

GAPDH Mouse Monoclonal (1C4)Antibody

MBS850080

1 mg

280 USD

monoclonal

unidentified

nonconjugated

[1C4]

Antibody

GAPDH Mouse Monoclonal (1C4)Antibody

[GAPDH]

[Glyceraldehyde-3-phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase; 38 kDa BFA-dependent ADP-ribosylation substrate; BARS-38; Peptidyl-cysteine S-nitrosylase GAPDH (EC:2.6.99.-)]

[GAPDH]

[LOC108351137; Gapdh; Gapd; GAPDH]

Monoclonal

IgG1

[1C4]

unknown

Human, Rat, Mouse, Rabbit, Sheep, Pig, Chicken

333

Liquid

Stable for 1 year at-20 degree C. For long term storage, Aliquot and store at-20 degree C or below. Avoid repeated freezing and thawing cycles.

Western Blot (WB)

WB: 1:5000

Western Blot

Immunogen: Full-length GAPDH protein of human.

Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively expressed in almost all tissues at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

122065190

P04797.3

P04797

35,828 Da

Alzheimer's Disease Pathway (83489); Alzheimer's Disease Pathway (509); Androgen Receptor Signaling Pathway (198529); Biosynthesis Of Amino Acids Pathway (791250); Biosynthesis Of Amino Acids Pathway (795174); Carbon Metabolism Pathway (816129); Carbon Metabolism Pathway (817567); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (434661); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (468196); Glycolysis (Embden-Meyerhof Pathway), Glucose = Pyruvate (434659)

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation ().

1 mg

280 USD