product summary
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company name :
MyBioSource
product type :
antibody
product name :
Mouse anti-Human CD48, Purified mAb
catalog :
MBS8500473
quantity :
50 Tests
price :
215 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
[4A156-4H9]
application :
immunocytochemistry, flow cytometry, FACS
more info or order :
MyBioSource product webpage
image
image 1 :
MyBioSource MBS8500473 image 1
Human peripheral blood lymphocytes analyzed with Purified CD48 mAb, followed by anti-mouse IgGs-FITC
product information
catalog number :
MBS8500473
products type :
Antibody
products full name :
Mouse anti-Human CD48, Purified mAb
products short name :
[CD48]
products gene name :
[CD48]
clonality :
Monoclonal
isotype :
IgG1
clone :
[4A156-4H9]
host :
Mouse
reactivity :
Human
form :
Phosphate-buffered solution, pH 7.4, containing 0.09% sodium azide
storage stability :
Store at 4 degree C. DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.
tested application :
Flow Cytometry (FC/FACS), Immunofluorescence (IF)
image1 heading :
Profile of peripheral blood lymphocytes analyzed by flow cytometry, FITC labeled goat anti-mouse IgG as secondary antibody staining.
other info2 :
Experimental Methods: 1.Take 100mul peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube; . 2.Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature; . 3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×); . 4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant; . 5.Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant; . 6.Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7.Repeat step 5. 8.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 degree C then measured). [PBS wash buffer: PBS +1% FBS +0.1% NaN3]. [Cell fixation: 2% formaldehyde solution]
products description :
4A156-4H9 reacts with blast-1, a 45 kDa GPI linked cell surface molecule. CD48 is expressed on peripheral blood lymphocytes, monocytes, or macrophages, but not on granulocytes and platelets nor on non-hematopoietic cells. CD48 binds to CD2 and plays a role as an accessory molecule in g/d T cell recognition and a/b T cell antigen recognition.* CD48 positive lymphocytes is decreased in patients with PNH (paroxysmal nocturnal haemoglobinuria)
products references :
1.Knapp, W., et al. (1989) Leucocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, New York. 2.Sandrin MS et al. (1993) J. Immunol. 151:4606. 3.GRiscelli-Bennaceur A et al. (1995) Blood 85:1354. 4.Flament, C et al. (1996) Hum. Immunol. 46:82.
size1 :
50 Tests
price1 :
215 USD
size2 :
100 Tests
price2 :
280
size3 :
500 Tests
price3 :
350
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

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Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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