antibody

BCL2 Antibody

MBS8500158

0.1 mL

310 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, immunohistochemistry

Antibody

BCL2 Antibody

[BCL2]

[BCL2; Bcl-2; ONCOGENE B-CELL LEUKEMIA 2; Apoptosis regulator Bcl-2]

[apoptosis regulator Bcl-2 isoform alpha; Apoptosis regulator Bcl-2; apoptosis regulator Bcl-2; BCL2, apoptosis regulator]

[BCL2]

[BCL2; BCL2; Bcl-2; PPP1R50]

polyclonal

IgG

rabbit

Human, Mouse, Rat

239

Affinity purification

Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

Western Blot (WB), Immunohistochemistry (IHC)

WB: 1:500-1:2000. IHC: 1:50-1:200. Western blot analysis of extracts of Jurkatcell line, using BCL2 antibody.Immunohistochemistry of paraffin-embedded Breast cancer using BCL2 Antibody.

Immunogen: Recombinant protein of human Bcl-2. Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

Bcl-2 exerts a survival function in response to a wide range of apoptotic stimuli through inhibition of mitochondrial cytochrome c release (1). It has been implicated in modulating mitochondrial calcium homeostasis and proton flux (2). Several phosphorylation sites have been identified within Bcl-2 including Thr56, Ser70, Thr74 and Ser87 (3). It has been suggested that these phosphorylation sites may be targets of the ASK1/MKK7/JNK1 pathway, and that phosphorylation of Bcl-2 may be a marker for mitotic events (4, 5). Mutation of Bcl-2 at Thr56 or Ser87 inhibits its anti-apoptotic activity during glucocorticoid-induced apoptosis of T lymphocytes (6). Interleukin 3 and JNK-induced Bcl-2 phosphorylation at Ser70 may be required for its enhanced anti-apoptotic functions (7).

1. Murphy, K.M. et al. (2000) Cell Death Differ 7, 102-11. 2. Zhu, L. et al. (1999) J Biol Chem 274, 33267-73. 3. Maundrell, K. et al. (1997) J Biol Chem 272, 25238-42. 4. Yamamoto, K. et al. (1999) Mol Cell Biol 19, 8469-78. 5. Ling, Y.H. et al. (1998) J Biol Chem 273, 18984-91. 6. Huang, S.T. and Cidlowski, J.A. (2002) FASEB J 16, 825-32. 7. Deng, X. et al. (2001) J Biol Chem 276, 23681-8.

72198189

NP_000624.2

NM_000633.2

P10415

26kDa

ATF-2 Transcription Factor Network Pathway (138006); Activation Of BAD And Translocation To Mitochondria Pathway (105659); Activation Of BH3-only Proteins Pathway (105658); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Apoptosis Pathway (198797); Apoptosis Pathway (83060); Apoptosis Pathway (470); Apoptosis Pathway (105648); Apoptosis Modulation And Signaling Pathway (198822)

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).

0.1 mL

310 USD