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5-Lipoxygenase Inhibitor Screening Kit (Fluorometric)

MBS846911

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Assay Kit

5-Lipoxygenase Inhibitor Screening Kit (Fluorometric)

[5-Lipoxygenase]

[5-lipoxygenase, partial; Arachidonate 5-lipoxygenase; arachidonate 5-lipoxygenase; arachidonate 5-lipoxygenase]

[ALOX5; ALOX5; 5-LO; 5LPG; LOG5; 5-LOX; LOG5; 5-LO; 5-lipoxygenase]

Human

50

Store at -20 degree C for 12 months.

Screening of potential inhibitors of 5-lipoxygenase

Typical Testing Data/Standard Curve (for reference only)

Fatty Acid Metabolism

Background/Introduction: 2-step plus is a two-step immunohistochemical broad spectrum detection reagent. It polymerizes monovalent Fab fragments of secondary antibody and enzyme, which replaces the secondary antibody and tertiary antibody in traditional method, can directly amplify the binding signal of antibody-antigen. This method not only retains the specific binding ability of antibody with antigen, but also can effectively avoid space steric hindrance caused by excessive polymer molecules. Compared with the traditional SP three-step method, this kit has the characteristics of simple, rapid, high-sensitivity. This system abandons the using of biotin, so it can avoid background staining by endogenous biotin. It can be used in IHC, in which the primary antibody is monoclonal/polyclonal antibody derived from goat. The unique polymer auxiliary agent for macromolecular detection system can detect the binding primary antibody better. Diluent for DAB concentrated solution has been provided in this kit to avoid the influence of the different water acidity and alkalinity on the DAB Chromogenic Agent.

187167

AAA63212.1

56,291 Da

Arachidonic Acid Metabolism Pathway (82991); Arachidonic Acid Metabolism Pathway (685553); Arachidonic Acid Metabolism Pathway (366); Eicosanoid Synthesis Pathway (198888); IL-5 Signaling Pathway (198770); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Ovarian Steroidogenesis Pathway (790011); Ovarian Steroidogenesis Pathway (795173); Selenium Pathway (198825)

This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

5-LO: arachidonate 5-lipoxygenase. Arachidonic acid promotes its phosphorylation by p38 MAPK-regulated MAPKAP kinases. Upregulated in glioblastoma multiforme and colon, pancreatic and bladder cancer. Protein type: EC 1.13.11.34; Lipid Metabolism - arachidonic acid; Lipid Metabolism - linoleic acid; Nuclear envelope; Oxidoreductase. Chromosomal Location of Human Ortholog: 10q11.21. Cellular Component: cytosol; extracellular region; extracellular space; nuclear envelope; nuclear envelope lumen; nuclear matrix; nuclear membrane; nucleoplasm. Molecular Function: arachidonate 5-lipoxygenase activity; iron ion binding; protein binding. Biological Process: leukotriene biosynthetic process; leukotriene metabolic process; lipoxygenase pathway; neutrophil degranulation. Disease: Asthma, Susceptibility To

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