catalog number :
MBS845430
products type :
Recombinant Protein
products full name :
Yellow Fluorescent Protein
products short name :
[Yellow Fluorescent Protein]
products name syn :
[YFP; Yellow Fluorescent Protein; Recombinant YFP Protein; Yellow Fluoroscent Protein]
other names :
[yellow fluorescent protein]
purity :
>=97% by SDS-PAGE and HPLC
storage stability :
Store at -80°C for long term storage.
tested application :
SDS-PAGE; HPLC
app notes :
The protein is suitable as control reagent for YFP expression studies or as labeling reagent. Applications include: Use as standards for SDS-PAGE, Western blot analysis of YFP transfected cells, label other proteins, calibration of fluorometers and flow cytometers, fluorescence microscope, or microinjection of YFP into cells and tissues, etc. The recombinant YFP is ideal for fusion tag applications and is perfect for triple labeling with EGFP (Cat.# MBS845247) and RFP (Cat.# MBS844904).
image1 heading :
SDS-PAGE
other info1 :
STLVTTLTYGAQCFAKYGPELKDFYKSCMPDGYVQERTITFEGDGNFKTRAEVTFENGSVYN RVKLNGQGFKKDGHVLGKNLEFNFTPHCLYIWGDQANHGLKSAFKICHEITGSKGDFIVADH TQMNTPIGGGPVHVPEYHHMSYHVKLSKDVTDHRDNMSLKETVRAVDCRKTYL
MRGSGSSGALLFHGKIPYVVEMEGNVDGHTFSIRGKGYG
DASVGKVDAQFICTTGDVPVPW
YFP AMINO ACID SEQUENCE:
other info2 :
Reconstitution: Reconstitute with dH2O to 1 mg/ml . Endotoxin Level: <=0.1 ng/ug
products description :
The recombinant YFP (Yellow Fluorescent Protein) is expressed and purified from transformed E. coli using a method that ensures high purity and maximal YFP fluorescence. Endotoxin has been removed, so the protein is suitable for in vivo injection or cell culture applications. The protein is a 26.4 kDa monomer with 238 amino acids, Ex./Em. = 525/538 nm, extinction coefficient 27765. (at 280 nm).
ncbi acc num :
AAQ96629.1