BRD2 bromodomain (1-455 aa) (His-Tag), human recombinant | MyBioSource MBS845316 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

BRD2 bromodomain (1-455 aa) (His-Tag), human recombinant

catalog :

MBS845316

quantity :

0.1 mg

price :

345 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS845316

products type :

Recombinant Protein

products full name :

BRD2 bromodomain (1-455 aa) (His-Tag), human recombinant

products short name :

[BRD2 bromodomain]

products name syn :

[RING3; RNF3; Bromodomain containing 4; D6S113E; FSH; FSRG1; NAT; RING3; RNF3]

other names :

[bromodomain-containing protein 2 isoform 1; Bromodomain-containing protein 2; bromodomain-containing protein 2; bromodomain containing 2; O27.1.1; Really interesting new gene 3 protein]

products gene name :

[BRD2]

other gene names :

[BRD2; BRD2; FSH; NAT; RNF3; FSRG1; RING3; D6S113E; KIAA9001; RING3]

uniprot entry name :

BRD2_HUMAN

sequence positions :

[1-455]

sequence length :

801

sequence :

MGSSHHHHHH SSGLVPRGSH MGSMLQNVTP HNKLPGEGNA GLLGLGPEAA APGKRIRKPS LLYEGFESPT MASVPALQLT PANPPPPEVS NPKKPGRVTN QLQYLHKVVM KALWKHQFAW PFRQPVDAVK LGLPDYHKII KQPMDMGTIK RRLENNYYWA ASECMQDFNT MFTNCYIYNK PTDDIVLMAQ TLEKIFLQKV ASMPQEEQEL VVTIPKNSHK KGAKLAALQG SVTSAHQVPA VSSVSHTALY TPPPEIPTTV LNIPHPSVIS SPLLKSLHSA GPPLLAVTAA PPAQPLAKKK GVKRKADTTT PTPTAILAPG SPASPPGSLE PKAARLPPMR RESGRPIKPP RKDLPDSQQQ HQSSKKGKLS EQLKHCNGIL KELLSKKHAA YAWPFYKPVD ASALGLHDYH DIIKHPMDLS TVKRKMENRD YRDAQEFAAD VRLMFSNCYK YNPPDHDVVA MARKLQDVFE FRYAKMPD

purity :

>90% by SDS-PAGE

form :

Formulation: 1 mg/ml in Phosphate buffer saline (pH 7.4) containing 10% glycerol and 1 mM DTT. Form: Liquid

storage stability :

Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

image1 heading :

Testing Data

other info1 :

Source: E.coli

other info2 :

Handling: Centrifuge the vial prior to opening.

products categories :

Epigenetics; Reader Domains; Bromodomains; BromodomainsProteins and Enzymes; Other Proteins & EnzymesProteins and Enzymes; Animal-Free Origin (AOF) Recombinant Proteins & EnzymesProteins and Enzymes; Proteins and Enzymes (A-Z)

products description :

Background: The acetylation of histone lysine residues plays a crucial role in the epigenetic regulation of gene transcription. A bromodomain is a protein domain that recognizes acetylated lysine residues such as those on the N-terminal tails of histones. This recognition is often a prerequisite for protein-histone association and chromatin remodeling. These domains function in the linking of protein complexes to acetylated nucleosomes, thereby controlling chromatin structure and gene expression. Thus, bromodomains serve as "readers" of histone acetylation marks regulating the transcription of target promoters. The BET family of proteins, defined by tandem Bromodomains and an Extra Terminal domain, include BRD2, BRD3, BRD4, and BRDT. The BET proteins play a key role in many cellular processes, including inflammatory gene expression, mitosis, and viral/host interactions. The isolated individual or tandem bromodomains of BRD2 and BRD4 have been shown to bind acetylated histone tails, serving to couple histone acetylation marks to the transcriptional regulation of target promoters. Small molecule inhibitors of these interactions hold promise as useful therapeutics for human disease. This protein can be used for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.

ncbi gi num :

164419758

ncbi acc num :

NP_001106653.1

ncbi gb acc num :

NM_001113182.2

uniprot acc num :

P25440

ncbi mol weight :

52.8 kDa (478 aa, 1-455 aa + His Tag), confirmed by MALDI-TOF.

ncbi pathways :

Regulation Of Retinoblastoma Protein Pathway (137916)

ncbi summary :

This gene encodes a transcriptional regulator that belongs to the BET (bromodomains and extra terminal domain) family of proteins. This protein associates with transcription complexes and with acetylated chromatin during mitosis, and it selectively binds to the acetylated lysine-12 residue of histone H4 via its two bromodomains. The gene maps to the major histocompatability complex (MHC) class II region on chromosome 6p21.3, but sequence comparison suggests that the protein is not involved in the immune response. This gene has been implicated in juvenile myoclonic epilepsy, a common form of epilepsy that becomes apparent in adolescence. Multiple alternatively spliced variants have been described for this gene. [provided by RefSeq, Dec 2010]

uniprot summary :

BRD2: an atypical protein kinase with two bromodomains that bind to acetylated lysine residues. Specifically interacts with transcriptionally active chromatin. Elevated protein kinase activity in leukemias. Transgenic mice overexpressing BRD2 in the lymphoid system develop diffuse large-cell lymphoma. Interacts with E2F1 and with histone H4 acetylated at Lys-13. May play a role in spermatogenesis or folliculogenesis. Genetic evidence links the BRD2 gene to both juvenile myoclonic epilepsy and photoparoxysomal responses. Two isoforms of the human protein are produced by alternative splicing. Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, atypical; ATYPICAL group; BRD family. Chromosomal Location of Human Ortholog: 6p21.3. Cellular Component: cytoplasm; nucleus. Molecular Function: protein binding; chromatin binding. Biological Process: regulation of transcription from RNA polymerase II promoter; nucleosome assembly; transcription, DNA-dependent; spermatogenesis; chromatin modification

size1 :

0.1 mg

price1 :

345 USD

more info or order :

MyBioSource product webpage