protein

Glucokinase, human liver, recombinant

MBS845289

0.01 mg

200 USD

Recombinant Protein

Glucokinase, human liver, recombinant

[Glucokinase]

[Glucokinase; Hexokinase type IV; HK IV; Hexokinase-4; HK4; Hexokinase-D]

[glucokinase isoform 1; Glucokinase; glucokinase; glucokinase (hexokinase 4); Hexokinase type IV; HK IV; Hexokinase-4; HK4; Hexokinase-D]

[GCK]

[GCK; GCK; GK; GLK; HK4; HHF3; HKIV; HXKP; LGLK; MODY2; FGQTL3; HK IV; HK4]

HXK4_HUMAN

E Coli

465

>=77% by SDS-PAGE

50 mM potassium phosphate pH 7.4, 50 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, and 2.5% glycerol. Appearance: Liquid

1 mg/ml

The protein is best stored in working aliquots at - 80°C. Avoid freeze/thaw cycles. Ships with Dry Ice.

SDS-PAGE; HPLC

Testing Data(TD)

Activity (Specifications/test method): 662 pmol/min/ug. One unit is defined as the amount of enzyme that will convert 1 pmol of NADP to NADPH at 30 degree C. Assay conditions: 25 mM HEPES, pH 7.5, 2 mM MgCl2, 1.0 mM DTT, 0.5 mM NADP, 2 mM ATP, 25 mM glucose, 100 ug/ml BSA, 20 units/ml glucose 6-phosphate dehydrogenase, and 10 nM human liver glucokinase at 30 degree C for 30 min. Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmol of NADP to NADPH at 30 degree C. Assay conditions: 25 mM HEPES, pH 7.5, 2 mM MgCl2, 1.0 mM DTT, 0.5 mM NADP, 2 mM ATP, 25 mM glucose, 100 ug/ml BSA, 20 units/ml glucose 6-phosphate dehydrogenase, and 10 nM human liver glucokinase at 30 degree C for 30 min. Handling: Centrifuge the vial prior to opening.

Proteins and Enzymes; Protein KinasesProteins and Enzymes; Proteins and Enzymes (A-Z) Proteins and Enzymes; Animal-Free Origin (AOF) Recombinant Proteins & EnzymesDiabetes, Obesity & Metabolic Syndrome; Other Products for Diabetes/Obesity Research

Background: Glucose is phosphorylated to glucose-6-phosphate by glucokinases. This gene is alternatively spliced to generate three different forms of the enzyme; one found in the pancreas and two found in the liver. The main function of this gene is to regulate carbohydrate metabolism. Recombinant human liver Glucokinase (Accession# NP_277042)) has a C-terminal FLAG tag and has 474 amino acid residues. It can be useful for studies including enzyme kinetics, activator screening and kinase selectivity.

4503951

NP_000153.1

NM_000162.3

P35557

53.4 kDa

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI). [provided by RefSeq, Apr 2009]

GK: a glycolytic enzyme that catalyzes glucose metabolism in the liver and pancreatic beta cells. Acts as a glucose sensor in beta cells. The first and rate-limiting step in glycosis, a pathway that produces energy in the form of ATP from glucose. Glucokinase traps glucose inside the cell by catalyzing its phosphorylation to produce glucose-6-phosphate. Has a lower affinity for glucose than the three other isozymes of hexokinase, allowing other organs such as the brain and muscles to have first call on glucose when its supply is limited. Unlike other hexokinases, glucokinase is not inhibited by glucose-6-phosphate. Glucokinase is found in the outer membrane compartment of mitochondria. May bind VDAC, suppressing mitochondrial function. Glucokinase transcription is induced by insulin, perhaps via the activation of Stat 5B. Mutant glucokinase causes a rare form of diabetes and may also play a role in type 2 diabetes. Three splice variant isoforms of human glucokinase have been described. Protein type: Mitochondrial; Carbohydrate Metabolism - starch and sucrose; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Kinase, other; Carbohydrate Metabolism - galactose; EC 2.7.1.2. Chromosomal Location of Human Ortholog: 7p15.3-p15.1. Cellular Component: nucleoplasm; mitochondrion; actin filament; basal cortex; cytosol; secretory granule. Molecular Function: protein binding; mannokinase activity; magnesium ion binding; fructokinase activity; ADP binding; glucokinase activity; protein phosphatase binding; ATP binding; glucose binding. Biological Process: positive regulation of glycogen biosynthetic process; glucose 6-phosphate metabolic process; lipid homeostasis; pathogenesis; glucose transport; glucose homeostasis; NADP metabolic process; cellular response to glucose starvation; elevation of cytosolic calcium ion concentration; detection of glucose; negative regulation of gluconeogenesis; regulation of glycolysis; transmembrane transport; second-messenger-mediated signaling; glycogen biosynthetic process; glycolysis; regulation of potassium ion transport; glucose metabolic process; positive regulation of insulin secretion; endocrine pancreas development; fructose 2,6-bisphosphate metabolic process; cellular response to insulin stimulus; hexose transport; positive regulation of glycolysis; carbohydrate phosphorylation; carbohydrate metabolic process; negative regulation of epinephrine secretion; cell glucose homeostasis; regulation of insulin secretion; positive regulation of phosphorylation. Disease: Hyperinsulinemic Hypoglycemia, Familial, 3; Diabetes Mellitus, Permanent Neonatal; Maturity-onset Diabetes Of The Young, Type 2; Diabetes Mellitus, Noninsulin-dependent

0.01 mg

200 USD