MMP-2, human recombinant | MyBioSource MBS845159 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

MMP-2, human recombinant

catalog :

MBS845159

quantity :

0.01 mg

price :

180 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS845159

products type :

Recombinant Protein

products full name :

MMP-2, human recombinant

products short name :

[MMP-2]

products name syn :

[72 kDa type IV collagenase; 72 kDa gelatinase; Gelatinase A; Matrix metalloproteinase-2; MMP-2; TBE-1; Cleaved into: PEX]

other names :

[72 kDa type IV collagenase isoform 2; 72 kDa type IV collagenase; 72 kDa type IV collagenase; matrix metallopeptidase 2; 72 kDa gelatinase; Gelatinase A; Matrix metalloproteinase-2; MMP-2]

products gene name :

[MMP2]

other gene names :

[MMP2; MMP2; CLG4; MONA; CLG4A; MMP-2; TBE-1; MMP-II; CLG4A; MMP-2]

uniprot entry name :

MMP2_HUMAN

sequence length :

610

purity :

>=95% by SDS-PAGE

form :

Lyophilized powder

storage stability :

The lyophilized protein is best-stored desiccated below 0°C. Reconstitute MMP2 should be stored in working aliquots at -20° C. For long term storage, it is recommended to add carrier protein (0.1% HAS or BSA)

other info1 :

Source: E.coli. Endotoxin Level: <0.1ng per ug of MMP-2

other info2 :

Solubility/Reconstitution Instructions: Reconstitute in sterile PBS to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use. Endotoxin Level: <0.1 ng/mug. Biological Activity: Biological activity of the protein has not been determined. Handling: Centrifuge the vial prior to opening.

products description :

Background: MMP-2 catalytic domain from human cDNA, expressed in E.coli and purified using proprietary technologies. The MMP-2 is useful as a standard in Western blot, etc.

ncbi gi num :

189217853

ncbi acc num :

NP_001121363.1

ncbi gb acc num :

NM_001127891.2

uniprot acc num :

P08253

ncbi mol weight :

40.0 kDa

ncbi pathways :

AGE/RAGE Pathway (698754); ATF-2 Transcription Factor Network Pathway (138006); Activation Of Matrix Metalloproteinases Pathway (1270258); Angiopoietin Receptor Tie2-mediated Signaling Pathway (137917); Axon Guidance Pathway (1270303); Bladder Cancer Pathway (83115); Bladder Cancer Pathway (527); Collagen Degradation Pathway (1270259); Degradation Of The Extracellular Matrix Pathway (1270257); Developmental Biology Pathway (1270302)

ncbi summary :

This gene is a member of the matrix metalloproteinase (MMP) gene family, that are zinc-dependent enzymes capable of cleaving components of the extracellular matrix and molecules involved in signal transduction. The protein encoded by this gene is a gelatinase A, type IV collagenase, that contains three fibronectin type II repeats in its catalytic site that allow binding of denatured type IV and V collagen and elastin. Unlike most MMP family members, activation of this protein can occur on the cell membrane. This enzyme can be activated extracellularly by proteases, or, intracellulary by its S-glutathiolation with no requirement for proteolytical removal of the pro-domain. This protein is thought to be involved in multiple pathways including roles in the nervous system, endometrial menstrual breakdown, regulation of vascularization, and metastasis. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]

uniprot summary :

MMP2: Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly- -Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Interacts (via the C-terminal hemopexin-like domains- containing region) with the integrin alpha-V/beta-3; the interaction promotes vascular invasion in angiogenic vessels and melamoma cells. Interacts (via the C-terminal PEX domain) with TIMP2 (via the C-terminal); the interaction inhibits the degradation activity. Interacts with GSK3B. Aspirin appears to inhibit expression. Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate. Inhibited by histatin-3 1/24 (histatin-5). Belongs to the peptidase M10A family. Protein type: Secreted; Protease; Secreted, signal peptide; Cell development/differentiation; Motility/polarity/chemotaxis; Apoptosis; EC 3.4.24.24. Chromosomal Location of Human Ortholog: 16q12.2. Cellular Component: proteinaceous extracellular matrix; extracellular space; sarcomere; mitochondrion; extracellular region; plasma membrane; nucleus. Molecular Function: protein binding; zinc ion binding; serine-type endopeptidase activity; metalloendopeptidase activity. Biological Process: axon guidance; collagen catabolic process; extracellular matrix disassembly; intramembranous ossification; extracellular matrix organization and biogenesis; cellular protein metabolic process; positive regulation of innate immune response; response to hypoxia; ephrin receptor signaling pathway; angiogenesis; proteolysis; blood vessel maturation; embryo implantation. Disease: Multicentric Osteolysis, Nodulosis, And Arthropathy

size1 :

0.01 mg

price1 :

180 USD

size2 :

0.05 mg

price2 :

345

size3 :

1 mg

price3 :

3630

more info or order :

MyBioSource product webpage