HMG-CoA Reductase Catalytic Domain (GST Tagged), human recombinant | MyBioSource MBS845121 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

HMG-CoA Reductase Catalytic Domain (GST Tagged), human recombinant

catalog :

MBS845121

quantity :

0.1 mg

price :

145 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS845121

products type :

Recombinant Protein

products full name :

HMG-CoA Reductase Catalytic Domain (GST Tagged), human recombinant

products short name :

[HMG-CoA Reductase Catalytic Domain]

products name syn :

[3-Hydroxy-3-methylglutaryl-CoA reductase; HMGR; HMGCR]

other names :

[3-hydroxy-3-methylglutaryl-Coenzyme A reductase isoform 1; 3-hydroxy-3-methylglutaryl-coenzyme A reductase; 3-hydroxy-3-methylglutaryl-Coenzyme A reductase; 3-hydroxy-3-methylglutaryl-CoA reductase]

products gene name :

[HMGCR]

other gene names :

[HMGCR; HMGCR; LDLCQ3; HMG-CoA reductase]

uniprot entry name :

HMDH_HUMAN

host :

E Coli

sequence length :

888

purity :

>=90%

form :

Lyophilized from proprietary buffer containing BSA. Appearance: Lyophilized

storage stability :

At -80 degree C. Shelf Life: 12 months

tested application :

SDS-PAGE

image1 heading :

Testing Data

other info2 :

Solubility/Reconstitution Instructions: Briefly spin down the vial and reconstitute in 50% glycerol in PBS to 0.5-1.0 mg/ml and store at -80 degree C. Activity (Specifications/test method): The specific activity was determined to be > 1.0 Unit/mg by MyBioSource's HMG-CoA reductase Activity/Inhibitor Screening Kit. Results: > 1.0 Unit/mg . Unit Definition: One unit is defined as the amount of enzyme required to convert 1.0 mumole of NADPH to NADP+ per minute at 37 degree C . Handling: Centrifuge the vial prior to opening.

products categories :

Proteins and Enzymes; Animal-Free Origin (AOF) Recombinant Proteins & EnzymesProteins and Enzymes; Proteins and Enzymes (A-Z)

products description :

Background: HMG-CoA reductase (3-hydroxy-3-methyl-glutaryl-CoA reductase or HMGR) (EC 1.1.1.34) is a transmembrane protein involved in the mevalonate pathway, the metabolic pathway that produces cholesterol from acetyl-CoA. In an NADPH-dependent reaction, HMG-CoA reductase reduces HMG-CoA to generate mevalonate and CoA. Since HMG-CoA reductase catalyzes the rate limiting step in cholesterol synthesis, it is a primary target of a group of cholesterol-lowering drugs known as statins. Inhibition of HMG-CoA reductase induces expression of LDL receptors in the liver, which lowers plasma concentration of cholesterol.

ncbi gi num :

4557643

ncbi acc num :

NP_000850.1

ncbi gb acc num :

NM_000859.2

uniprot acc num :

P04035

ncbi mol weight :

75.9 kDa (426-888 aa, NT GST Tag)

ncbi pathways :

AMPK Signaling Pathway (198868); AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); Activation Of Gene Expression By SREBF (SREBP) Pathway (1270039); Bile Secretion Pathway (193146); Bile Secretion Pathway (193095); Biosynthesis Of Antibiotics Pathway (1144997); C5 Isoprenoid Biosynthesis, Mevalonate Pathway (413387); C5 Isoprenoid Biosynthesis, Mevalonate Pathway (468288); Cholesterol Biosynthesis Pathway (198809)

ncbi summary :

HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

uniprot summary :

HMGCR: the rate-limiting enzyme for cholesterol synthesis. Regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by this reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Protein type: Membrane protein, multi-pass; Endoplasmic reticulum; EC 1.1.1.34; Secondary Metabolites Metabolism - terpenoid backbone biosynthesis; Membrane protein, integral; Motility/polarity/chemotaxis; Oxidoreductase. Chromosomal Location of Human Ortholog: 5q13.3-q14. Cellular Component: peroxisomal membrane; endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane. Molecular Function: protein binding; protein homodimerization activity; hydroxymethylglutaryl-CoA reductase (NADPH) activity; hydroxymethylglutaryl-CoA reductase activity; coenzyme binding. Biological Process: coenzyme A metabolic process; myoblast differentiation; negative regulation of MAP kinase activity; ubiquinone metabolic process; response to ethanol; isoprenoid biosynthetic process; negative regulation of vasodilation; positive regulation of smooth muscle cell proliferation; visual learning; positive regulation of skeletal muscle development; cellular lipid metabolic process; cholesterol biosynthetic process; protein tetramerization; response to nutrient; positive regulation of stress-activated MAPK cascade; aging

size1 :

0.1 mg

price1 :

145 USD

size2 :

0.5 mg

price2 :

375

more info or order :

MyBioSource product webpage