Active Recombinant Human beta-Secretase 1 - BACE-1 | MyBioSource MBS845073 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Active Recombinant Human beta-Secretase 1 - BACE-1

catalog :

MBS845073

quantity :

0.005 mg

price :

245 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS845073

products type :

Recombinant Protein

products full name :

Active Recombinant Human beta-Secretase 1 - BACE-1

products short name :

[beta-Secretase 1 - BACE-1]

products name syn :

[Beta-secretase 1; Aspartyl protease 2; ASP2; Beta-site amyloid precursor protein cleaving enzyme 1; Beta-site APP cleaving enzyme 1; Memapsin-2; Membrane-associated aspartic protease 2; Aspartic-like protease 56 kDa; Aspartyl protease 1; Beta-site amyloid precursor protein cleaving enzyme 2; Down region aspartic protease; Memapsin-1; Membrane-associated aspartic protease 1; Theta-secretase]

other names :

[beta-secretase 1 isoform F; Beta-secretase 1; beta-secretase 1; beta-site APP-cleaving enzyme 1; Aspartyl protease 2; ASP2; Asp 2; Beta-site amyloid precursor protein cleaving enzyme 1; Beta-site APP cleaving enzyme 1; Memapsin-2; Membrane-associated aspartic protease 2]

products gene name :

[BACE1]

other gene names :

[BACE1; BACE1; ASP2; BACE; HSPC104; BACE; KIAA1149; ASP2; Asp 2; Beta-site APP cleaving enzyme 1]

uniprot entry name :

BACE1_HUMAN

host :

Insect cell

sequence length :

376

specificity :

> or = 120 x 10 3 units/mg

purity :

> or = 90% by SDS-PAGE

form :

Supplied in PBS with 50% glycerol

storage stability :

Stable for 1 year at –70°C. Avoid multiple freeze/thaw cycles as activity may decrease.

tested application :

SDS-PAGE; HPLC

app notes :

Active recombinant human BACE-1 is useful in studying enzyme regulation, determining target substrates, screening BACE inhibitors, or as a positive control in BACE activity assays. MBS, Inc. recombinant BACE-1 produced in insect cells is about 200 times higher in specific activity relative to that produced in E. coli.

image1 heading :

Testing Data

other info2 :

Solubility/Reconstitution Instructions: Use 20 ul sterile D.I. water to dissolve 10 ug of protein for a final conc of 0.5 ug/ul. For further dilution, use 10 mM Tris, pH 8.0. Biological Activity: MyBioSource's recombinant human beta-Secretase/BACE-1 is biologically active as determined by using the beta-Secretase Activity Assay Kit. Unit Definition: One unit is defined as the amount of enzyme that produces 1 pmol/min EDANS by hydrolyzing the substrate BVT9964 (NH2-Arg-Glu(EDANS)-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys(DABCYL)-Arg-COOH) at 37 degree C in 100 mM acetate buffer pH 4.0. Handling: Centrifuge the vial prior to opening.

products categories :

Proteins and Enzymes; Proteins and Enzymes (A-Z)Proteins and Enzymes; Animal-Free Origin (AOF) Recombinant Proteins & Enzymes

products description :

Beta-Secretase, also called BACE1 (beta-site of APP cleaving enzyme) or memapsin-2 is an aspartic-acid protease important in the pathogenesis of Alzheimer's disease, and in the formation of myelin sheaths in peripheral nerve cells. The transmembrane protein contains two active site aspartate residues in its extracellular protein domain.

ncbi gi num :

333755186

ncbi acc num :

NP_001193978.1

ncbi gb acc num :

NM_001207049.1

uniprot acc num :

P56817

ncbi mol weight :

47 kDa (mature protease domain, 46-460aa)

ncbi pathways :

Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448)

ncbi summary :

Cerebral deposition of amyloid beta peptide is an early and critical feature of Alzheimer's disease. Amyloid beta peptide is generated by proteolytic cleavage of amyloid precursor protein (APP) by two proteases, one of which is the protein encoded by this gene. The encoded protein, a member of the peptidase A1 protein family, is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. Multiple transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, May 2011]

uniprot summary :

BACE: an integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. Cleaves APP at the amino terminus of the A-beta peptide sequence, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated carboxy-terminal fragment which is later release by gamma-secretase. Four splice-variant isoforms have been described. Protein type: Protease; Membrane protein, integral; EC 3.4.23.46. Chromosomal Location of Human Ortholog: 11q23.2-q23.3. Cellular Component: Golgi apparatus; multivesicular body; cytoplasmic vesicle membrane; cell surface; integral to plasma membrane; axon; endoplasmic reticulum; late endosome; integral to membrane; plasma membrane; trans-Golgi network; endosome. Molecular Function: protein binding; enzyme binding; beta-amyloid binding; beta-aspartyl-peptidase activity; aspartic-type endopeptidase activity. Biological Process: beta-amyloid metabolic process; membrane protein ectodomain proteolysis; proteolysis

size1 :

0.005 mg

price1 :

245 USD

size2 :

0.05 mg

price2 :

655

size3 :

1 mg

price3 :

9025

more info or order :

MyBioSource product webpage