Human CellExp HMGB1 / HMG1, human recombinant | MyBioSource MBS844990 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Human CellExp HMGB1 / HMG1, human recombinant

catalog :

MBS844990

quantity :

0.01 mg

price :

210 USD

more info or order :

MyBioSource product webpage

image

image 1 :

SDS-PAGE (4-20%) of Recombinant HMGB1: Recombinant protein loaded under reducing conditions and stained with Coomassie Blue. The protein shows a predicted MW of 32 kDa

image 2 :

Biological activity: Measured by its binding ability in a functional ELISA HMGB1 is binding to immobilized human AGER-Fc tag protein (100 ug/well) with a linear range of 0.31-2.5 ug/ml.

product information

catalog number :

MBS844990

products type :

Recombinant Protein

products full name :

Human CellExp HMGB1 / HMG1, human recombinant

products short name :

[HMGB1/HMG1]

products name syn :

[HMGB1; HMG1; HMG3; SBP-1]

other names :

[high mobility group protein B1; High mobility group protein B1; high mobility group protein B1; high mobility group box 1; High mobility group protein 1; HMG-1]

products gene name :

[HMGB1]

other gene names :

[HMGB1; HMGB1; HMG1; HMG3; SBP-1; HMG1; HMG-1]

uniprot entry name :

HMGB1_HUMAN

host :

HEK 293 cells (Met1-Glu 215)

sequence length :

215

purity :

> 95% by SDS-PAGE

form :

Lyophilized from 0.22 um filtered solution in PBS. Trehalose (5%) was added as a protectant before lyophilization.

storage stability :

Store at -20°C. After reconstitution, aliquot and store at -20°C and use within 3 months. Avoid repeated freezing and thawing.

tested application :

SDS-PAGE; HPLC

image1 heading :

SDS-PAGE

image2 heading :

ELISA

other info1 :

Endotoxin Level: <1 EU/ug by LAL method

other info2 :

Reconstitution: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4. Do not vortex. Store the solution in aliquots at -20°C for long term storage. Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized human AGER-Fc tag protein (100 ul/well) can bind to HMGB1 in a linear range of 0.31-2.5 ug/ml.

products categories :

Cytokines, Growth Factors & Hormones; Human cell expressed recombinant proteins Proteins and Enzymes; Human cell expressed recombinant proteins Proteins and Enzymes; Proteins and Enzymes (A-Z)Cytokines, Growth Factors & Hormones; Cytokines & Growth Factor

products description :

High-mobility group protein B1 (HMGB1), also known as high-mobility group protein 1 (HMG-1) and amphoterin, is a member of the HMGB family consisting of three members, HMGB1, HMGB2 and HMGB3. HMGB1 is a non-histone architectural chromosomal protein ubiquitously present in all vertebrate nuclei and binds double-stranded DNA without sequence specificity. The mechanism of inflammation and damage is binding to TLR4, which mediates HMGB1-dependent activation of macrophage cytokine release. This positions HMGB1 at the intersection of sterile and infectious inflammatory responses. HMGB1 has been studied as a DNA vaccine adjuvant and a target for cancer therapy.

ncbi gi num :

4504425

ncbi acc num :

NP_002119.1

ncbi gb acc num :

NM_002128.4

uniprot acc num :

P09429

ncbi mol weight :

The protein is fused with 6×His tag at the C-terminus, has a calculated MW of 25 kDa. The predicted N-terminus is Met1. DTT-reduced Protein migrates as 32 kDa and 35 kDa due to glycosylation.

uniprot summary :

HMGB1: DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. Component of the RAG complex composed of core components RAG1 and RAG2, and associated component HMGB1 or HMGB2. Belongs to the HMGB family. Protein type: Nuclear receptor co-regulator; DNA repair, damage. Chromosomal Location of Human Ortholog: 13q12. Cellular Component: nucleoplasm; extracellular space; cell surface; transcriptional repressor complex; extracellular region; condensed chromosome; nucleus. Molecular Function: protein binding; RAGE receptor binding; double-stranded DNA binding; damaged DNA binding; cytokine activity; chromatin binding; transcription factor activity; transcription factor binding; DNA bending activity; single-stranded DNA binding; chemoattractant activity. Biological Process: negative regulation of transcriptional preinitiation complex assembly; DNA topological change; V(D)J recombination; positive regulation of apoptosis; apoptosis; positive regulation of caspase activity; base-excision repair, DNA ligation; negative regulation of transcription from RNA polymerase II promoter; DNA recombination; regulation of transcription from RNA polymerase II promoter; chromatin remodeling; myeloid dendritic cell activation; inflammatory response to antigenic stimulus; positive chemotaxis; dendritic cell chemotaxis; innate immune response; DNA ligation during DNA repair; positive regulation of transcription from RNA polymerase II promoter; DNA fragmentation during apoptosis; neurite development; cell structure disassembly during apoptosis; positive regulation of DNA binding

size1 :

0.01 mg

price1 :

210 USD

more info or order :

MyBioSource product webpage