BRD4 bromodomains 1 and 2 (49-460 aa) (GST-tagged), Human recombinant | MyBioSource MBS844747 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

BRD4 bromodomains 1 and 2 (49-460 aa) (GST-tagged), Human recombinant

catalog :

MBS844747

quantity :

0.02 mg

price :

245 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS844747

products type :

Recombinant Protein

products full name :

BRD4 bromodomains 1 and 2 (49-460 aa) (GST-tagged), Human recombinant

products short name :

[BRD4 bromodomains 1 and 2]

products name syn :

[Bromodomain containing 4; HUNK1; MCAP; BRD4 bromodomains 1 and 2 (49-460 aa) (GST-tagged); Human recombinant]

other names :

[bromodomain-containing protein 4 isoform short; Bromodomain-containing protein 4; bromodomain-containing protein 4; bromodomain containing 4; Protein HUNK1]

products gene name :

[BRD4]

other gene names :

[BRD4; BRD4; CAP; MCAP; HUNK1; HUNKI; HUNK1]

uniprot entry name :

BRD4_HUMAN

purity :

>=90% by SDS-PAGE

form :

50 mM Tris, pH 7.5, containing 500 mM sodium chloride, 5% glycerol, and 5 mM beta-mercaptoethanol.

storage stability :

Store at -80 degree C. Stable for: >=6 months

tested application :

SDS-PAGE; HPLC

image1 heading :

Testing Data

other info1 :

Source: E Coli

other info2 :

Handling: Centrifuge the vial prior to opening.

products categories :

Epigenetics; Bromodomains; Bromodomain Proteins (New category)Epigenetics; Others; Recombinant ProteinsProteins and Enzymes; Animal-Free Origin (AOF) Recombinant Proteins & EnzymesProteins and Enzymes; Proteins and Enzymes (A-Z)

products description :

Background: The acetylation of histone lysine residues plays a crucial role in the epigenetic regulation of gene transcription. A bromodomain is a protein domain that recognizes acetylated lysine residues such as those on the N-terminal tails of histones. This recognition is often a prerequisite for protein-histone association and chromatin remodeling. These domains function in the linking of protein complexes to acetylated nucleosomes, thereby controlling chromatin structure and gene expression. Thus, bromodomains serve as "readers" of histone acetylation marks regulating the transcription of target promoters. The BET family of proteins, defined by tandem Bromodomains and an Extra Terminal domain, include BRD2, BRD3, BRD4, and BRDT. The BET proteins play a key role in many cellular processes, including inflammatory gene expression, mitosis, and viral/host interactions. The isolated individual or tandem bromodomains of BRD2 and BRD4 have been shown to bind acetylated histone tails, serving to couple histone acetylation marks to the transcriptional regulation of target promoters. Small molecule inhibitors of these interactions hold promise as useful therapeutics for human disease.

ncbi gi num :

7657218

ncbi acc num :

NP_055114.1

ncbi gb acc num :

NM_014299.2

uniprot acc num :

O60885

ncbi mol weight :

73.4 kDa (49-460 aa + GST Tag)

ncbi summary :

The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008]

uniprot summary :

BRD4: an atypical protein kinase of the Brd family. Contains a double bromodomain. Binds to chromatin and regulates cell cycle progression at multiple stages. Associates with chromosomes during mitosis. The cognate gene is the chromosome 19 target of translocation t(15;19)(q13;p13.1), resulting in the BRD4-NUT oncogene identified in a lethal carcinoma of young people. Binds to papillomavirus E2 protein and is required for its transforming ability. Two alternatively spliced isoforms have been described. Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, atypical; ATYPICAL group; BRD family. Chromosomal Location of Human Ortholog: 19p13.1. Cellular Component: nucleoplasm; transcription elongation factor complex b; condensed nuclear chromosome; cytoplasm; nuclear chromatin; chromosome; nucleus. Molecular Function: protein binding; DNA binding; p53 binding; chromatin binding. Biological Process: chromatin remodeling; G1/S-specific transcription in mitotic cell cycle; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; viral reproduction; positive regulation of RNA elongation from RNA polymerase II promoter; regulation of inflammatory response; positive regulation of transcription from RNA polymerase II promoter; inner cell mass cell proliferation; protein amino acid phosphorylation; response to DNA damage stimulus; positive regulation of DNA binding; chromosome segregation

size1 :

0.02 mg

price1 :

245 USD

size2 :

0.1 mg

price2 :

745

more info or order :

MyBioSource product webpage