GSK-3a-GST Fusion Protein | MyBioSource MBS844576 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

GSK-3a-GST Fusion Protein

catalog :

MBS844576

quantity :

0.1 mg

price :

200 USD

more info or order :

MyBioSource product webpage

image

image 1 :

GSK-3alpha-GST Fusion Protein

product information

catalog number :

MBS844576

products type :

Recombinant Protein

products full name :

GSK-3a-GST Fusion Protein

products short name :

[GSK-3a-GST Fusion Protein]

products name syn :

[GSK; Glycogen synthase kinase-3 (GSK-3)]

other names :

[glycogen synthase kinase-3 alpha; Glycogen synthase kinase-3 alpha; glycogen synthase kinase-3 alpha; glycogen synthase kinase 3 alpha; Serine/threonine-protein kinase GSK3A (EC:2.7.11.1)]

products gene name :

[GSK3A]

other gene names :

[GSK3A; GSK3A; GSK-3 alpha]

uniprot entry name :

GSK3A_HUMAN

host :

E Coli

sequence length :

483

form :

Lyophilized powder without additives.

concentration :

2 mg/ml

storage stability :

Store at -20 degree C. For long term storage, aliquot and freeze at -70 degree C. Avoid repeated freeze/defrost cycles.

tested application :

SDS-PAGE; HPLC

image1 heading :

Test Data

other info1 :

Preparation: GSK-3alpha (P49840) was expressed in E. coli. It is expressed as a recombinant fusion protein of GSK-3 residues (amino acid 12-30) surrounding GSK-3 (Ser21) and glutathione S-transferase (GST). The protein was purified by affinity chromatography. Reconstition: Reconstitute in sterile H2O

other info2 :

Activity (Specifications/test method): GSK-3alpha fusion protein at a concentration of 2 ug/20 ul reaction was phosphorylated in an in vitro kinase assay with 200 uM ATP. After 30 minutes incubation at 30 degree C, phosphorylation was detected by Western blot with phospho-specific GSK-3alpha antibodies. Handling: Centrifuge the vial prior to opening.

products description :

Background: GSK-3 is one of the Akt substrates. It can be phosphorylated by Akt at Ser21 of GSK-3alpha or Ser9 of GSK-3beta. The phosphorylation of GSK negatively regulates its kinase activity. The recombinant GSK-3alpha fusion protein serves as a useful substrate for assaying Akt kinase activity.

ncbi gi num :

49574532

ncbi acc num :

NP_063937.2

ncbi gb acc num :

NM_019884.2

uniprot acc num :

P49840

ncbi mol weight :

37.0 kDa

ncbi pathways :

AKT Phosphorylates Targets In The Cytosol Pathway (1269189); Adaptive Immune System Pathway (1269171); B Cell Receptor Signaling Pathway (198909); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class I PI3K Signaling Events Mediated By Akt Pathway (138020); Constitutive Signaling By AKT1 E17K In Cancer Pathway (1268881); DAP12 Interactions Pathway (1269283); DAP12 Signaling Pathway (1269284); Disease Pathway (1268854)

ncbi summary :

This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease. [provided by RefSeq, Oct 2011]

uniprot summary :

GSK3A: a proline-directed protein kinase of the GSK family. Implicated in the control of several regulatory proteins including glycogen synthase, Myb, and c-Jun. GSK3 and GSK3 have similar functions. GSK3 phophorylates tau, the principal component of neurofibrillary tangles in Alzheimer disease and is required for maximal production of amyloid plaque peptides by secretase. A GSK3 promoter SNP effects progression of bipolar disorder. The GSK3 inhibitor, lithium, is used to treat bipolar disorder and is seen to block plaque formation. GSK3 generally opposes the action of insulin, and GSK3 hyperactivity is thought to contribute to insulin resistant (type II) diabetes. GSK3 also negatively regulates cardiac hypertrophy. A tumor suppressor role is indicated by the oncogenic potential of stabilized -catenin mutants that lack GSK3 phosphorylation sites. Inhibitor: AR-A014418. Protein type: Kinase, protein; Protein kinase, CMGC; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.26; CMGC group; GSK family; GSK subfamily. Chromosomal Location of Human Ortholog: 19q13.2. Cellular Component: beta-catenin destruction complex; cytosol. Molecular Function: protein serine/threonine kinase activity; protein binding; tau-protein kinase activity; ATP binding. Biological Process: glycogen metabolic process; proteasomal ubiquitin-dependent protein catabolic process; genetic imprinting; nerve growth factor receptor signaling pathway; negative regulation of insulin receptor signaling pathway; protein amino acid phosphorylation; negative regulation of transferase activity; positive regulation of cAMP biosynthetic process; negative regulation of glycogen biosynthetic process; epidermal growth factor receptor signaling pathway; nervous system development; cell migration; negative regulation of TOR signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; Wnt receptor signaling pathway; unfolded protein response; positive regulation of peptidyl-serine phosphorylation; negative regulation of glucose import; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; cellular response to insulin stimulus; positive regulation of protein catabolic process; regulation of systemic arterial blood pressure; insulin receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of heart contraction

size1 :

0.1 mg

price1 :

200 USD

more info or order :

MyBioSource product webpage