protein

Benzonase, E. Coli Recombinant

MBS844281

5000 Units

140 USD

Recombinant Protein

Benzonase, E. Coli Recombinant

[Benzonase]

[Endoribonuclease]

[ssRNA-specific endoribonuclease; 16S rRNA 3' end maturation and quality control co-endoribonuclease working with RNase R; rRNA transcription antitermination factor; Endoribonuclease YbeY; ssRNA-specific endoribonuclease; 16S rRNA 3' end maturation and quality control co-endoribonuclease working with RNase R; rRNA transcription antitermination factor]

[YbeY]

[ybeY; ybeY; ECK0651; JW0656]

YBEY_ECOLI

E Coli

155

>=98% by SDS-PAGE.

Liquid; 250 U/ul in 50% glycerol

Store at -20°C in aliquots.

Removal of DNA/RNA from proteins; Purification of recombinant protein from inclusion bodies; Viscosity reduction of protein samples, etc.

Testing Data

Working Conditions: Optimal pH 8-8.5, functional pH 6-10. •Optimal temperature 35°C - 42°C, functional 0°C to 42°C. •Magnesium (1-2 mM) is required for activity. In the presence of 1 mM gCl2, activity is reduced 30% by 1mM EDTA or completely nactivated by 0.1M EDTA. Activity is reduced 75% by 0.1 M CaCl2or 1M aCl. •Under standard assay conditions, 1 mM iodoacetate had no effect on enzymatic activity. 1 mM mercaptoethanol and maleic acid educed tivity 5 to 10%. 10 mM p-Chloromercurybenzoate completely inactivates the enzyme. 0.64 M beta-mercaptoethanol in the presence of 2 rea slightly decreases enzymatic activity. 4 to 7 M Urea increases enzymatic activity. Unit definition: One unit is defined as the change of A260 = 1 (equivalent to 37 ug of DNA) when sonicated salmon sperm DNA is digested with the enzyme for 30 min at 37°C at pH 8.0.

Molecular Biology Tools; Molecular Biology Proteins/EnzymesProteins and Enzymes; Animal-Free Origin (AOF) Recombinant Proteins & EnzymesProteins and Enzymes; Proteins and Enzymes (A-Z)

Background: Golden Nuclease is a recombinant form of Serratia macescens extracellular endonuclease produced in Escherichia coli. Golden Nuclease is a homodimer with monomer molecular masses about 30 kDa. Two disulfide bonds found in the nuclease are crucial to its activity and stability. The enzyme is a non-specific nuclease with high specific activity, which degrades both single- and double-stranded nucleic acids in any form (single stranded, double stranded, linear, circular and supercoiled). It hydrolyzes internal phosphodiester bonds present between the nucleotides to 5'-phosphorylated oligonucleotides of 3-8 bases in length.

16128642

NP_415192.1

NC_000913.3

P0A898

~ 30 kDa

AFMB Structural Genomics target Number 122 (http://afmb.cnrs-mrs.fr/article171.html). [More information is available at EcoGene: EG13655]. YbeY shows similarity to metal-dependent hydrolases. [More information is available at EcoCyc: G6362].

Single strand-specific metallo-endoribonuclease involved in late-stage 70S ribosome quality control and in maturation of the 3' terminus of the 16S rRNA. Acts together with the RNase R to eliminate defective 70S ribosomes, but not properly matured 70S ribosomes or individual subunits, by a process mediated specifically by the 30S ribosomal subunit. Involved in the processing of 16S, 23S and 5S rRNAs, with a particularly strong effect on maturation at both the 5'-and 3'-ends of 16S rRNA as well as maturation of the 5'-end of 23S and 5S rRNAs.

5000 Units

140 USD

25000 Units

290