protein

Eosinophil Peroxidase, Human Eosinophils

MBS844058

0.01 mg

300 USD

Protein

Eosinophil Peroxidase, Human Eosinophils

[Eosinophil Peroxidase, Human Eosinophils]

[EPO; EPER; EPP]

[eosinophil peroxidase preproprotein; Eosinophil peroxidase; eosinophil peroxidase; eosinophil peroxidase]

[EPX]

[EPX; EPX; EPO; EPP; EPX-PEN; EPER; EPO; EPP; EPO]

PERE_HUMAN

Human eosinophils

715

> or = 95% by SDS-PAGE

Lyophilized from 20 mM NaH2PO4, pH 7.5, and 150 mM NaCI

At -20 degree C. After reconsitution, aliquot and store at -20 degree C.

SDS-PAGE; HPLC

SDS-PAGE

Source: Human Eosinophils (Prepared from whole blood shown to be non-reactive for HBsAg, anti-HCV, anti- HBc, and negative for anti-HIV 1 & 2 by FDA approved tests).

Solubility/Reconstitution Instructions: Reconstitute in deionized H2O. Aliquot and store at -20 degree C. Avoid freeze-thaw cycles. Activity (Specifications/test method): >=180 units per mg protein after lyophilization. Unit Definition: One unit of activity is defined as the amount of enzyme which will decompose one micromole of hydrogen peroxide per minute at 25 degree C, pH 6.0. Handling: Centrifuge the vial prior to opening. Reconstitution: Deionized Water.

Proteins and Enzymes; Native ProteinsProteins Other Proteins & Enzymes and Proteins & Enzymes (A-Z)

Background: Native, human eosinophil peroxidase is a secreted hemoprotein that catalyzes oxidations of various substrates by hydrogen peroxide. Together with other leukocyte-derived enzymes, such as Myeloperoxidase, EPO plays an important role in the pathogenesis of asthma by mediating oxidative events that lead to the production of ROS (reactive oxygen species) and RNS (reactive nitrogen species).

4503595

NP_000493.1

NM_000502.4

P11678

Asthma Pathway (83120); Asthma Pathway (532)

This gene is a member of the peroxidase gene family and is expressed in eosinophils. The encoded precursor protein is processed into covalently attached heavy and light chains to form the mature enzyme, which functions as an oxidant. The enzyme is released at sites of parasitic infection or allergen stimulation to mediate lysis of protozoa or parasitic worms. The gene is found in a cluster of three peroxidase genes at chromosome 17q23. Mutations in this gene result in eosinophil peroxidase deficiency. [provided by RefSeq, Sep 2009]

EPX: Mediates tyrosine nitration of secondary granule proteins in mature resting eosinophils. Shows significant inhibitory activity towards Mycobacterium tuberculosis H37Rv by inducing bacterial fragmentation and lysis. Defects in EPX are the cause of eosinophil peroxidase deficiency (EPD). EPD is an autosomal recessive defect where anomalous eosinophils are characterized by nuclear hypersegmentation, hypogranulation, and negative peroxidase and phospholipid staining. Belongs to the peroxidase family. XPO subfamily. Protein type: Oxidoreductase; EC 1.11.1.7. Chromosomal Location of Human Ortholog: 17q23.1. Molecular Function: peroxidase activity; metal ion binding; heme binding. Biological Process: positive regulation of interleukin-4 production; negative regulation of interleukin-10 production; hydrogen peroxide catabolic process; negative regulation of interleukin-5 production; response to oxidative stress; defense response to nematode. Disease: Eosinophil Peroxidase Deficiency

0.01 mg

300 USD

0.05 mg

820