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Tyrosinase Inhibitor Screening Kit (Colorimetric)

MBS841896

100 Assays

780 USD

Assay Kit

Tyrosinase Inhibitor Screening Kit (Colorimetric)

[Tyrosinase]

[tyrosinase; Tyrosinase; tyrosinase; tyrosinase; LB24-AB; Monophenol monooxygenase; SK29-AB; Tumor rejection antigen AB]

[TYR; TYR; ATN; CMM8; OCA1; OCA1A; OCAIA; SHEP3]

TYRO_HUMAN

529

At -20 degree C. Shelf Life: 12 months

Screening/studying/characterizing tyrosinase inhibitors

Metabolism Assays; Other Metabolism-Related Assays; Metabolism Assays; Metabolism Assays (A-Z)

Background/Introduction: Tyrosinase or polyphenol oxidase (EC 1.14.18.1), is an oxidoreductase that participates in the biosynthesis of melanin, a ubiquitous biological pigment found in hair, eyes, skin, etc. Inhibition of tyrosinase has been a long-time target in the skin health research, cosmetics and agricultural industries because of its role in browning reactions in skin pigmentation and during fruit harvesting and handling. Skin whitening and bleaching products utilize natural or synthetic tyrosinase inhibitors in order to lighten the skin color. Polyphenols, benzaldehyde derivatives, long-chain lipids, steroids, and natural compounds have been used as tyrosinase inhibitors. Tyrosinase catalyzes the oxidation of tyrosine, producing a chromophore that can be detected at 510 nm. In the presence of Kojic Acid, a reversible inhibitor of tyrosinase, the rate of oxidation of the substrate is decreased. The kit provides a rapid, simple, sensitive, and reliable test suitable for high-throughput screening of tyrosinase inhibitors. The assay is also adaptable to a 384-well format.

4507753

NP_000363.1

NM_000372.4

42,914 Da

(S)-reticuline Biosynthesis Pathway (547499); (S)-reticuline Biosynthesis II Pathway (138845); Catecholamine Biosynthesis, Tyrosine = Dopamine = Noradrenaline = Adrenaline Pathway (413357); Catecholamine Biosynthesis, Tyrosine = Dopamine = Noradrenaline = Adrenaline Pathway (468235); Dopamine Metabolism Pathway (920995); L-dopachrome Biosynthesis Pathway (545322); L-dopachrome Biosynthesis Pathway (545496); Melanin Biosynthesis Pathway (1270185); Melanogenesis Pathway (83092); Melanogenesis Pathway (504)

The enzyme encoded by this gene catalyzes the first 2 steps, and at least 1 subsequent step, in the conversion of tyrosine to melanin. The enzyme has both tyrosine hydroxylase and dopa oxidase catalytic activities, and requires copper for function. Mutations in this gene result in oculocutaneous albinism, and nonpathologic polymorphisms result in skin pigmentation variation. The human genome contains a pseudogene similar to the 3' half of this gene. [provided by RefSeq, Oct 2008]

TYR: This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6-dihydroxyindole to indole-5,6 quinone. Increased expression after UVB irradiation. Belongs to the tyrosinase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Oxidoreductase; Membrane protein, integral; EC 1.14.18.1; Cofactor and Vitamin Metabolism - riboflavin; Amino Acid Metabolism - tyrosine. Chromosomal Location of Human Ortholog: 11q14.3. Cellular Component: perinuclear region of cytoplasm; lysosome; melanosome membrane; cytoplasm; integral to membrane; melanosome; Golgi-associated vesicle. Molecular Function: protein binding; copper ion binding; protein homodimerization activity; protein heterodimerization activity; monophenol monooxygenase activity. Biological Process: cell proliferation; visual perception; thymus development; melanin biosynthetic process from tyrosine; eye pigment biosynthetic process. Disease: Albinism, Ocular, With Sensorineural Deafness; Albinism, Oculocutaneous, Type Ib; Albinism, Oculocutaneous, Type Ia; Skin/hair/eye Pigmentation, Variation In, 3

100 Assays

780 USD