other

Caspase-3 Fluorometric Assay Kit

MBS841894

25 Assays

230 USD

Assay Kit

Caspase-3 Fluorometric Assay Kit

[Caspase-3]

[caspase-3 preproprotein; Caspase-3; caspase-3; caspase 3, apoptosis-related cysteine peptidase; Apopain; Cysteine protease CPP32; CPP-32]

[CASP3; CASP3; CPP32; SCA-1; CPP32B; CPP32; CASP-3; CPP-32; SCA-1]

CASP3_HUMAN

277

At -20 degree C. Shelf Life: 12 months

Typical Testing Data/Standard Curve (for reference only)

Samples: Cell and tissue lysates

Features & Benefits: Simple one-step procedure; takes 1-2 hours. Fast and convenient. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-3/CPP32 activity

Apoptosis & Related Products; Caspases; Caspase-3; Caspase-3 Assay Kits

Product Information: Caspase-3/CPP32 Activity Fluorometric Assay Kit: Simple & Convenient Fluorometric Assay to Measure Caspase-3/CPP32 Activity within 1-2 hrs. Kit Size: 25, 100, 200 and 400 assays. Background: Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The CPP32/Caspase-3 Fluorometric Protease Assay Kit provides a simple and convenient means for assaying the DEVD-dependent caspase activity. The assay is based on detection of cleavage of substrate DEVD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). DEVD-AFC emits blue light (lambdamax = 400 nm); upon cleavage of the substrate by CPP32 or related caspases, free AFC emits a yellow-green fluorescence (?max = 505 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-3/CPP32 activity. Summary: Detection method- fluorecence (400 nm excitation filter and 505 nm emission filter) . Species reactivity- Mammalian . Kit size- Convenient sizes (25, 100, 200, 400 assays) . Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

14790119

NP_004337.2

NM_004346.3

31,608 Da

AGE/RAGE Pathway (698754); Activation Of DNA Fragmentation Factor Pathway (1270293); Activation Of Caspases Through Apoptosome-mediated Cleavage Pathway (1270283); Alpha6-Beta4 Integrin Signaling Pathway (198807); Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Amyotrophic Lateral Sclerosis (ALS) Pathway (920975)

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq, Jul 2008]

CASP3: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a 216-Asp- -Gly-217 bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Interacts with BIRC6/bruce. Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. Inhibited by isatin sulfonamides. Belongs to the peptidase C14A family. Protein type: Apoptosis; Motility/polarity/chemotaxis; EC 3.4.22.56; Protease. Chromosomal Location of Human Ortholog: 4q34. Cellular Component: nucleoplasm; plasma membrane; nucleus; cytosol. Molecular Function: cyclin-dependent protein kinase inhibitor activity; peptidase activity; protein binding; cysteine-type endopeptidase activity; aspartic-type endopeptidase activity. Biological Process: extracellular matrix organization and biogenesis; nerve growth factor receptor signaling pathway; apoptosis; positive regulation of apoptosis; negative regulation of activated T cell proliferation; heart development; negative regulation of B cell proliferation; regulation of caspase activity; proteolysis; neuron differentiation; extracellular matrix disassembly; sensory perception of sound; B cell homeostasis; positive regulation of neuron apoptosis; response to wounding; erythrocyte differentiation; T cell homeostasis; DNA fragmentation during apoptosis; response to UV; cell structure disassembly during apoptosis; release of cytochrome c from mitochondria; cell fate commitment; negative regulation of cyclin-dependent protein kinase activity; keratinocyte differentiation; neuron apoptosis; induction of apoptosis via death domain receptors; caspase activation via cytochrome c; platelet formation; response to DNA damage stimulus; induction of apoptosis by oxidative stress; negative regulation of apoptosis

25 Assays

230 USD

100 Assays

465

200 Assays

635

400 Assays

1005