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DPP4 Activity Fluorometric Assay Kit

MBS841858

100 Assays

495 USD

Assay Kit

DPP4 Activity Fluorometric Assay Kit

DPP4

dipeptidyl peptidase 4; Dipeptidyl peptidase 4; dipeptidyl peptidase 4; dipeptidyl-peptidase 4; ADABP; Adenosine deaminase complexing protein 2; ADCP-2

DPP4

DPP4; DPP4; CD26; ADABP; ADCP2; DPPIV; TP103; ADCP2; CD26; ADCP-2; DPP IV

DPP4_HUMAN

766

At -20 degree C. Shelf Life: 12 months

Samples: Cell and tissue culture lysates, plasma, serum, other biological fluids, cell culture medium, etc.

Metabolism Assays; Other Metabolism-Related AssaysMetabolism Assays; Metabolism Assays (A-Z)Signal Transduction; Assay Kits

Product Information: Dipeptidyl peptidase-4 (DPP4) Activity Assay Kit: High-Throughput Suitable Fluorometric Assay for detecting DPP4 Activity in Cells, Tissues etc. Simple, Reliable, Highly Sensitive. Detection Limit: 3 uU. 100 Assays. Background: Dipeptidyl peptidase-4 (DPP4), also known as adenosine deaminase complexing protein 2 or CD26 (cluster of differentiation 26) is a protein that, in humans, is encoded by the DPP4 gene. The substrates of CD26/DPP4 are proline (or alanine) containing peptides and include growth factors, chemokines, neuropeptides, and vasoactive peptides. DPP4 plays a major role in glucose metabolism. It is responsible for the degradation of incretins such as GLP-1 and hence its inhibition by drugs such as Sitagliptin have been used for treatment of diabetes mellitus type 2. DPP4 also appears to work as a suppressor in the development of cancer and tumors. In MyBioSource's DPP4 Activity Assay Kit, DPP4 cleaves a substrate to release a quenched fluorescent group, AMC (7-Amino-4-Methyl Coumarin), (Ex/Em = 360/460 nm). This assay is rapid, simple, sensitive, and reliable, as well as, suitable for high throughput activity screening of DPP4. This kit detects DPP4 activity as low as 3 uU per well. Summary: Detection method- Fluorescence (Ex/Em 360/460 nm) . Species reactivity- Mammalian . Applications- This kit detects DPP4 activity as low as 3 uU per well.

18765694

NP_001926.2

NM_001935.3

88,279 Da

Incretin Synthesis, Secretion, And Inactivation Pathway (1268750); Metabolism Of Proteins Pathway (1268677); Peptide Hormone Metabolism Pathway (1268746); Protein Digestion And Absorption Pathway (172847); Protein Digestion And Absorption Pathway (171868); Synthesis, Secretion, And Inactivation Of Glucagon-like Peptide-1 (GLP-1) Pathway (1268751); Synthesis, Secretion, And Inactivation Of Glucose-dependent Insulinotropic Polypeptide (GIP) Pathway (1268752)

The protein encoded by this gene is identical to adenosine deaminase complexing protein-2, and to the T-cell activation antigen CD26. It is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. [provided by RefSeq, Jul 2008]

DPP4: Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF- kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline. Belongs to the peptidase S9B family. DPPIV subfamily. Protein type: Cell adhesion; Cell surface; Membrane protein, integral; Protease; EC 3.4.14.5. Chromosomal Location of Human Ortholog: 2q24.3. Cellular Component: intercellular canaliculus; cell surface; focal adhesion; membrane; endocytic vesicle; lysosomal membrane; lamellipodium; apical plasma membrane; integral to membrane; plasma membrane; lipid raft. Molecular Function: identical protein binding; protein binding; protein homodimerization activity; protease binding; dipeptidyl-peptidase activity; serine-type peptidase activity; serine-type endopeptidase activity; receptor binding. Biological Process: regulation of cell-cell adhesion mediated by integrin; T cell activation; positive regulation of cell proliferation; T cell costimulation; response to hypoxia; endothelial cell migration; proteolysis

100 Assays

495 USD