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product summary
company name :
MyBioSource
product type :
other
product name :
GST Colorimetric Activity Assay Kit
catalog :
MBS841810
quantity :
100 Assays
price :
495 USD
product information
catalog number :
MBS841810
products type :
Assay Kit
products full name :
GST Colorimetric Activity Assay Kit
products short name :
[GST Colorimetric Activity Assay Kit]
storage stability :
At -20 degree C. Shelf Life: 12 months
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids
other info2 :
Features & Benefits: Simple procedure; takes only ~1-1.5 hours. Fast and convenient
products categories :
Cell Damage & Oxidative Stress; Glutathione & Related ProductsCell Damage & Oxidative Stress; Cell Damage & Oxidative Stress Assay KitsMetabolism Assays; Oxidative StressMetabolism Assays; Metabolism Assays (A-Z)
products description :
Product Information: Glutathione S-Transferase (GST) Colorimetric Activity Assay Kit: Detect GST Activity in Crude Cell Lysate or Purified Protein Fraction and Detect or Quantitate GST-tagged fusion recombinant protein. Simple, Absorbance-based in vitro Assay. Detection Limit < 1 mU. Background: Glutathione S-transferase (GST) is a family of enzymes that play an important role in detoxification of xenobiotics. GST catalyzes the formation of the thiol group of glutathione to electrophilic xenobiotics. It utilizes glutathione to scavenge potentially toxic compounds including those produced as a result of oxidative stress and is part of the defense mechanism against the mutagenic, carcinogenic and toxic effects of such compounds. The GST Colorimetric Activity Assay Kit is based upon the GST-catalyzed reaction between GSH and the GST substrate, CDNB (1-chloro-2,4-dinitrobenzene, which has the broadest range of isozyme detectability (e.g., alpha-, mu-, pi-, and other GST isoforms). Under certain conditions, the interaction between glutathione and CDNB is totally dependent on the presence of active GST. The GST-catalyzed formation of GS-DNB produces a dinitrophenyl thioether which can be detected by spectrophotometer at 340 nm. One unit of GST activity is defined as the amount of enzyme producing 1 umol of GS-DNB conjugate/min under the conditions of the assay. The kit can detect GST activity in crude cell lysate or purified protein fraction, and also quantitate GST-tagged fusion protein. Detect limit: Active GST < 4 mU. Summary: Detection method- Absorbance (380 nm) . Species reactivity- Mammalian . Applications- The kit can detect GST activity in crude cell lysate or purified protein fraction, and also quantitate GST-tagged fusion protein.
size1 :
100 Assays
price1 :
495 USD
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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