other

Cobalt Colorimetric Assay Kit

MBS841804

100 Assays

440 USD

Assay Kit

Cobalt Colorimetric Assay Kit

[Cobalt]

At -20 degree C. Shelf Life: 12 months

Typical Testing Data/Standard Curve (for reference only)

Metabolism Assays; Inorganic IonsMetabolism Assays; Metabolism Assays (A-Z)

Background: Cobalt is a hard, lustrous, grey metal, a chemical element (symbol Co, atomic number 27 and atomic mass 58.9). Cobalt is an essential trace-element for all multicellular organisms as the active center of coenzymes called cobalamins [1]. These include vitamin B-12 which is essential for plants and animals. Cobalt is also an active nutrient for bacteria, algae, and fungi, and may be a necessary nutrient for all life. Cobalt, particularly the Co2+ species, forms complexes with a large number of organic and inorganic molecules with a range of extinction coefficients in the visible range. The Cobalt Assay kit takes advantage of the reaction of Co+2 with mercaptoethanol in basic media. Under the condition, Cobalt forms a complex with a strong absorbance band at 475 nm. The metal ions Fe2+, Cu2+, Ni2+, Zn2+, Mn2+, exhibit less than 10% interference at this wavelength. The kit provides a quick simple accurate method of quantitating Co2+ in a variety of samples. The assay is linear in the range 3 to 50 nmol (0.18-3 ug) Cobalt, with a detection limit of ~ 3 nmol (or ~15 uM, 0.18 ug).

100 Assays

440 USD