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Acid Sphingomyelinase Activity Colorimetric Assay Kit

MBS841772

100 Assays

470 USD

Assay Kit

Acid Sphingomyelinase Activity Colorimetric Assay Kit

[Acid Sphingomyelinase]

[acid sphingomyelinase; Sphingomyelin phosphodiesterase; sphingomyelin phosphodiesterase; sphingomyelin phosphodiesterase 1, acid lysosomal; Acid sphingomyelinase; aSMase]

[SMPD1; SMPD1; ASM; NPD; ASMASE; ASM; aSMase]

ASM_HUMAN

629

At -80 degree C. Shelf Life: 12 months

Measurement of ASMase activity in various tissues/cell extracts. Screening of ASMase inhibitors

Typical Testing Data/Standard Curve (for reference only)

Samples: Tissue Extracts, Cell Lysate, Cell Culture Media And Other Biological Fluids Samples. Detection Range: 0.1 mg/ml-5 mg/ml

Metabolism Assays; Lipids Metabolism; Metabolism Assays; Metabolism Assays (A-Z)

Background/Introduction: Sorbitol is one of the 6 carbon sugar alcohols. It is commonly used as an artificial sweetener, as a laxative and in cosmetics as a humectant and thickening agent. Sorbitol is produced naturally in a variety of fruits. It can be produced in humans in small amounts by the reduction of glucose by aldose reductase. Due to its poor ability to diffuse across the cell membrane, sorbitol can be trapped in cells and is believed to be one of the causes of damage (due to osmotic effects) in diabetes. Sorbitol can react with Cu2+ under alkaline solution development of intense color with an absorbance at 655 nm.

179095

AAA58377.1

69,624 Da

Ceramide Signaling Pathway (138023); FAS (CD95) Signaling Pathway (138081); Glycosphingolipid Metabolism Pathway (1270099); IL2 Signaling Events Mediated By PI3K Pathway (137938); Lysosome Pathway (99052); Lysosome Pathway (96865); Metabolic Pathways (132956); Metabolism Pathway (1269956); Metabolism Of Lipids And Lipoproteins Pathway (1270001); Ovarian Infertility Genes Pathway (198801)

The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2010]

SMPD1: Converts sphingomyelin to ceramide. Also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol. Isoform 2 and isoform 3 have lost catalytic activity. Monomer. Belongs to the acid sphingomyelinase family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.1.4.12; Phosphodiesterase; Lipid Metabolism - sphingolipid. Chromosomal Location of Human Ortholog: 11p15.4-p15.1. Cellular Component: extracellular space; lysosomal lumen; lysosome; plasma membrane; endosome; lamellar body. Molecular Function: protein binding; sphingomyelin phosphodiesterase activity; hydrolase activity, acting on glycosyl bonds. Biological Process: response to drug; nervous system development; negative regulation of MAP kinase activity; sphingolipid metabolic process; sphingomyelin metabolic process; positive regulation of apoptosis; ceramide biosynthetic process; glycosphingolipid metabolic process; sphingomyelin catabolic process; response to cocaine; signal transduction; positive regulation of protein amino acid dephosphorylation. Disease: Niemann-pick Disease, Type B; Niemann-pick Disease, Type A

100 Assays

470 USD