Caspase-9 Antibody | MyBioSource MBS841177 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Caspase-9 Antibody

catalog :

MBS841177

quantity :

0.1 mg

price :

305 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, cow

application :

western blot

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS841177

products type :

Antibody

products full name :

Caspase-9 Antibody

products short name :

[Caspase-9]

products name syn :

[CASP-9; EC 3.4.22.62; ICE-like apoptotic protease 6,ICE-LAP6,Apoptotic protease Mch-6,Apoptotic protease-activating factor 3; Apaf-3]

other names :

[caspase-9; Caspase-9; caspase-9; caspase 9, apoptosis-related cysteine peptidase; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; APAF-3]

other gene names :

[CASP9; CASP9; MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6; MCH6; CASP-9; APAF-3; ICE-LAP6]

uniprot entry name :

CASP9_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat, Hamster, Bovine, Rabbit, Pig

sequence length :

266

specificity :

This antibody detects proform (~46kDa), intermediate (~33-36 kDa), and the fully cleaved (~20-25kDa) caspase-9

purity :

Affinity purified

form :

0.5 mg/ml affinity purified rabbit polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5mM EDTA and 0.03% proclin

storage stability :

Store at -20°C. For long term storage, aliquot and freeze at -70°C. Avoid repeated freeze/defrost cycles.

tested application :

Western Blot (WB)

app notes :

Western blotting (0.5-4 ug/ml). However, the optimal conditions should be determined individually. Blocking peptide is available seperately ( MBS843499 )

image1 heading :

Testing Data

other info1 :

Immunogen: Synthetic peptide surrounding amino acid 290 of human caspase-9

other info2 :

Handling: The antibody solution should be gently mixed before use.

products description :

Background: Caspase-9 is one of the most important caspases among the caspase family members. Caspase-9 is synthesized as inactive pro-enzyme that is processed in cells undergoing apoptosis. The processed caspase-9 consists of large (32-35 kD) and small (10 kD) subunits which associate to form an active enzyme. Activated caspase-9 in turn cleaves and activates caspase-3, one of the proteases responsible for the proteolytic cleavage of many key proteins in apoptosis.

ncbi gi num :

6518502

ncbi acc num :

BAA87905.1

ncbi mol weight :

36,564 Da

ncbi summary :

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]

uniprot summary :

CASP9: a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alternative splicing results in two isoforms. Protein type: EC 3.4.22.62; Protease; Apoptosis. Chromosomal Location of Human Ortholog: 1p36.21. Cellular Component: mitochondrion; nucleus; cytosol; apoptosome. Molecular Function: peptidase activity; protein binding; cysteine-type endopeptidase activity; enzyme activator activity; protein kinase binding; SH3 domain binding. Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; apoptosis; positive regulation of apoptosis; DNA damage response, signal transduction; response to lipopolysaccharide; proteolysis; response to estradiol stimulus; response to antibiotic; DNA damage response, signal transduction resulting in induction of apoptosis; positive regulation of neuron apoptosis; response to cobalt ion; innate immune response; platelet formation; caspase activation via cytochrome c; response to DNA damage stimulus; aging

size1 :

0.1 mg

price1 :

305 USD

more info or order :

MyBioSource product webpage