antibody

Caspase-9 Antibody

MBS840560

0.03 mg

150 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

Antibody

Caspase-9 Antibody

[Caspase-9]

[CASP-9,EC 3.4.22.62,ICE-like apoptotic protease 6; ICE-LAP6,Apoptotic protease Mch-6,Apoptotic protease-activating factor 3; Apaf-3]

[caspase9; Caspase-9; caspase-9; caspase 9; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; APAF-3]

[Casp9; Casp9; Mch6; APAF-3; CASP-9; AI115399; AW493809; ICE-LAP6; Caspase-9; Mch6; CASP-9; APAF-3; ICE-LAP6]

CASP9_MOUSE

Polyclonal

IgG

Rabbit

Human, Mouse, Rat

454

The antibody detects 45 kDa procaspase-9, 35/37 kDa cleaved immediate fragments and the 17-25 kDa fully cleaved caspase-9.

Affinity purified

0.5mg/ml affinity purified rabbit anti-caspase-9 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.03% proclin.

Store at -20°C. For long term storage, aliquot and freeze at -70°C. Avoid repeated freeze/defrost cycles.

Western Blot (WB)

The antibody can be used for Western blotting (0.5 -4 ug/ml). However, the optimal conditions should be determined individually. Jurkat cell lysate can be used as a positive control.

Testing Data

Immunogen: Synthetic peptide surrounding amino acid 290 of mouse caspase-9

Handling: The antibody solution should be gently mixed before use.

Caspase family of cysteine proteases plays a key role in apoptosis. Caspase-9 is one of the most important caspases among the caspase family members. Upon induction of apoptosis, cytochrome c released from mitochondria associates with procaspase-9/Apaf-1. The complex processes procaspase-9 into a large subunit (35 kDa or 17-25 kDa) and small (10 kDa) by self-cleavage at D315. Activated caspase-9 further cleaves other caspase members including caspase-3, one of the proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. In addition to self-cleavage, procaspase-9 can also be cleaved in vivo by caspase-3 at D330. The process served as a positive feedback to amplify the apoptotic signal in caspase activation pathway.

6440942

BAA86895

49,979 Da

This gene is part of a family of caspases, aspartate-specific cysteine proteases well studied for their involvement in immune and apoptosis signaling. This protein, the initiator caspase, is activated after cytochrome c release from mitochondria and targets downstream effectors. In mouse, deficiency of this gene can cause perinatal lethality. This protein may have a role in normal brain development. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Apr 2013]

CASP9: a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alternative splicing results in two isoforms. Protein type: Protease; EC 3.4.22.62; Apoptosis. Cellular Component: mitochondrion; cytoplasm; intracellular; nucleus; cytosol; apoptosome. Molecular Function: peptidase activity; protein binding; hydrolase activity; cysteine-type endopeptidase activity; SH3 domain binding; protein kinase binding; cysteine-type peptidase activity. Biological Process: caspase activation; regulation of apoptosis; DNA damage response, signal transduction resulting in induction of apoptosis; positive regulation of apoptosis; apoptosis; positive regulation of neuron apoptosis; DNA damage response, signal transduction; proteolysis; response to organic cyclic substance; response to DNA damage stimulus; response to UV

0.03 mg

150 USD

0.1 mg

300