antibody

JNK Antibody

MBS840351

0.03 mg

150 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, immunohistochemistry, immunoprecipitation

Antibody

JNK Antibody

[JNK]

[JNK1; JNK 1; JNK1 antibody,SARP; JNK1A2; JNK; JAK 1A; JAK1A; Stress activated protein kinase]

[mitogen-activated protein kinase 8 JNK1 beta2; Mitogen-activated protein kinase 8; mitogen-activated protein kinase 8; mitogen-activated protein kinase 8; JNK-46; Stress-activated protein kinase 1c; SAPK1c; Stress-activated protein kinase JNK1; c-Jun N-terminal kinase 1]

[JNK]

[MAPK8; MAPK8; JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2; JNK1; PRKM8; SAPK1; SAPK1C; MAP kinase 8; MAPK 8; SAPK1c]

MK08_HUMAN

Polyclonal

IgG

Rabbit

Human, Mouse, Rat

427

Affinity purified

100 ug (0.5 mg/ml) affinity purified rabbit anti-JNK polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.

Store at -20°C. For long term storage, aliquot and freeze at -70°C. Avoid repeated freeze/defrost cycles.

Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Western blotting (0.5-4 ug/ml. Immunoprecipitation (10-20 ug/ml). Immunohistochemistry (10-20 ug/ml). However, the optimal conditions should be determined individually. Blocking peptide (MBS840351) is available separately.

Testing Data

Immunogen: Synthetic peptide surrounding amino acid 412 of human JNK-1

Handling: The antibody solution should be gently mixed before use.

Background: The JNK pathway is activated by largely distinct stimuli including inflammatory cytokines (such as TNF-alpha and IL-1), UV light, inhibitors of protein synthesis and osmotic stress. Activated MEKK1 phosphorylates SEK1 (also known as MKK4), which in turn activates SAPK (also known as JNK). JNK binds tightly to the N-terminal region of c-Jun and ATF-2, and phosphorylates c-Jun at Ser63 and Ser73 and ATF-2 at Thr69 and Thr71.

513788279

NP_001265476.1

NM_001278547.1

P45983

AGE/RAGE Pathway (698754); ATF-2 Transcription Factor Network Pathway (138006); Activated TLR4 Signalling Pathway (1269236); Activation Of BH3-only Proteins Pathway (1270270); Activation Of BIM And Translocation To Mitochondria Pathway (1270274); Activation Of BMF And Translocation To Mitochondria Pathway (1270275); Activation Of The AP-1 Family Of Transcription Factors Pathway (1269218); Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Angiopoietin Receptor Tie2-mediated Signaling Pathway (137917)

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Five alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jun 2013]

JNK1: a protein kinase of the MAPK family that is potently activated by a variety of environmental stresses, including UV and gamma radiation, ceramides, pro-inflammatory cytokines and, in some instances, by growth factors and GPCR agonists. Substrates include a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2, thus regulating AP-1 transcriptional activity. In T- cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells. Binds to at least four scaffolding proteins, JIP-1, -2, -3 and -4. Activity increased in obesity. Inhibition or mouse knockout increases insulin sensitivity. Part of NFB pathway involved in inflammation and cancer, and signals downstream of Ras, though possibly as an apoptotic negative regulator of growth. Four alternatively-spliced isoforms have been described. Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.24; Protein kinase, CMGC; Kinase, protein; CMGC group; MAPK family; JNK subfamily; MAPK/JNK subfamily. Chromosomal Location of Human Ortholog: 10q11.22. Cellular Component: nucleoplasm; mitochondrion; cytosol; nucleus. Molecular Function: protein serine/threonine kinase activity; protein binding; histone deacetylase regulator activity; enzyme binding; histone deacetylase binding; JUN kinase activity; ATP binding. Biological Process: nerve growth factor receptor signaling pathway; apoptosis; positive regulation of apoptosis; rhythmic process; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; protein amino acid phosphorylation; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; regulation of protein localization; regulation of transcription factor activity; JNK cascade; response to stress; negative regulation of protein binding; toll-like receptor 4 signaling pathway; response to UV; ossification; regulation of histone deacetylation; MyD88-independent toll-like receptor signaling pathway; peptidyl-threonine phosphorylation; regulation of circadian rhythm; toll-like receptor 2 signaling pathway; JUN phosphorylation; MyD88-dependent toll-like receptor signaling pathway; peptidyl-serine phosphorylation; response to cadmium ion; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; negative regulation of apoptosis

0.03 mg

150 USD

0.1 mg

290