ALDH2 Antibody (NT) | MyBioSource MBS840238 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

ALDH2 Antibody (NT)

catalog :

MBS840238

quantity :

0.1 mL

price :

290 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot analysis in A549, HepG2 and NCI-H92 cell lysates and mouse liver and lung and rat liver tissue lysates (35 µg/lane).

image 2 :

Flurorescent confocal image of A549 cell stained with ALDH2 Antibody (N-term). A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ALDH2 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). ALDH2 immunoreactivity is localized to Mitochondrion significantly.

product information

catalog number :

MBS840238

products type :

Antibody

products full name :

ALDH2 Antibody (NT)

products short name :

[ALDH2]

products name syn :

[ALDH2; ALDM; Aldehyde dehydrogenase; mitochondrial; ALDH class 2; ALDH-E2; ALDHI.]

other names :

[aldehyde dehydrogenase, mitochondrial isoform 1; Aldehyde dehydrogenase, mitochondrial; aldehyde dehydrogenase, mitochondrial; aldehyde dehydrogenase 2 family (mitochondrial); ALDH class 2; ALDH-E2; ALDHI]

products gene name :

[ALDH2]

other gene names :

[ALDH2; ALDH2; ALDM; ALDHI; ALDH-E2; ALDM]

uniprot entry name :

ALDH2_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

517

purity :

This antibody is purified through a protein A column, followed by peptide affinity purification.

form :

Liquid . Supplied in PBS with 0.09(w/v)sodium azide.

storage stability :

Mantain refrigerated at 2-8°C for up to 6 months. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), Immunofluorescence (IF)

app notes :

Western blot: ~1:1000, IF: ~1:10-1:50.

image1 heading :

Western Blot WB

image2 heading :

Fluorescent FL

other info1 :

Immunogen: This ALDH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 52-81 amino acids from the N-terminal region of human ALDH2. Positive Control: Western blot: A549, HepG2 and NCI-H92 cell lysates and mouse liver and lung and rat liver tissue lysates, IF: A549 cells.

other info2 :

Handling: The antibody solution should be gently mixed before use.

products categories :

Antibodies & Supporting Tools; Antibodies for Metabolic Processes (NEW)Diabetes, Obesity & Metabolic Syndrome; Antibodies (NEW) Antibodies & Supporting Tools; Primary Antibodies (A-Z)

products description :

Polyclonal Antibody to detect ALDH2 in human, mouse and rat samples. Background: ALDH2 (Aldehyde dehydrogenase 2 family) belongs to the aldehyde dehydrogenase family which catalyze the chemical transformation from acetaldehyde to acetic acid and is the second enzyme of the major oxidative pathway of alcohol metabolism. Aldehyde dehydrogenases (ALDHs) mediate NADP+-dependent oxidation of aldehydes into acids during detoxification of alcohol-derived acetaldehyde; lipid peroxidation; and metabolism of corticosteroids, biogenic amines and neurotransmitters. ALDH1A1, also designated retinal dehydrogenase 1 (RalDH1 or RALDH1); aldehyde dehydrogenase family 1 member A1; aldehyde dehydrogenase cytosolic; ALDHII; ALDH-E1 or ALDH E1, is a retinal dehydrogenase that participates in the biosynthesis of retinoic acid (RA). The major liver isoform ALDH1 localizes to cytosolic space, while ALDH2 localizes to the mitochondria. The ALDH1A2 (RALDH2, RALDH2-T) gene produces three different transcripts and also catalyzes the synthesis of RA from retinaldehyde. ALDH2 is present in most Caucasians, yet is absent in 50% of Asians. The absence of this enzyme has been linked to alcohol intolerance; and thusly, a reduced risk for alcoholism-related liver disease.

ncbi gi num :

25777732

ncbi acc num :

NP_000681.2

ncbi gb acc num :

NM_000690.3

uniprot acc num :

P05091

ncbi mol weight :

56.38 kDa

ncbi pathways :

Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); Ascorbate And Aldarate Metabolism Pathway (82932); Ascorbate And Aldarate Metabolism Pathway (293); Biological Oxidations Pathway (1270189); Biosynthesis Of Antibiotics Pathway (1144997); Ethanol Oxidation Pathway (1270206); Fatty Acid Omega Oxidation Pathway (198915); Fatty Acid Degradation Pathway (82935); Fatty Acid Degradation Pathway (296)

ncbi summary :

This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Mar 2011]

uniprot summary :

ALDH2: This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Mar 2011]. Protein type: Other Amino Acids Metabolism - beta-alanine; Amino Acid Metabolism - histidine; Carbohydrate Metabolism - butanoate; Amino Acid Metabolism - lysine degradation; Carbohydrate Metabolism - propanoate; Amino Acid Metabolism - arginine and proline; Lipid Metabolism - glycerolipid; Mitochondrial; Oxidoreductase; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Secondary Metabolites Metabolism - limonene and pinene degradation; Carbohydrate Metabolism - pyruvate; Amino Acid Metabolism - tryptophan; Lipid Metabolism - fatty acid; EC 1.2.1.3; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - ascorbate and aldarate. Chromosomal Location of Human Ortholog: 12q24.2. Cellular Component: mitochondrial matrix. Molecular Function: aldehyde dehydrogenase (NAD) activity; electron carrier activity; aldehyde dehydrogenase [NAD(P)+] activity. Biological Process: synaptic transmission; ethanol catabolic process; xenobiotic metabolic process; carbohydrate metabolic process; alcohol metabolic process; ethanol oxidation; neurotransmitter biosynthetic process. Disease: Alcohol Sensitivity, Acute

size1 :

0.1 mL

price1 :

290 USD

more info or order :

MyBioSource product webpage