antibody

FasL Antibody

MBS840226

0.03 mg

150 USD

polyclonal

rabbit

nonconjugated

mouse, rat

Antibody

FasL Antibody

[FasL]

[FASLG; FASL; FasL; CD95L; APT1LG1; CD178; APTL; TNFSF6]

[tumor necrosis factor ligand superfamily member 6 isoform 1; Tumor necrosis factor ligand superfamily member 6; tumor necrosis factor ligand superfamily member 6; Fas ligand (TNF superfamily, member 6); CD95 ligand; CD95-L]

[FasL]

[Fasl; Faslg; gld; CD178; CD95L; Fas-L; Faslg; CD95-L; Tnfsf6; APT1LG1; Apt1lg1; Cd95l; Fasl; gld; Tnfsf6; CD95-L; Fas ligand; FasL; sFasL; APL; FasL ICD; SPA]

TNFL6_MOUSE

Polyclonal

IgG

Rabbit

Mouse, Rat

279

Detects ~31 kDa FasL in samples of mouse and rat origins. A ~70 kDa band, possible represents thr FasL and Fas complex can also be detected.

0.5 mg/mL affinity purified rabbit polyclonal antibody in phosphate buffered saline (PBS),pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.03% proclin.

Store at -20°C. For long term storage, aliquot and freeze at -70°C. Avoid repeated freeze/defrost cycles.

Western Blot (WB)

Western blotting (0.5-4 ug/ml). However, the optimal conditions should be determined individually.Blocking peptide (MBS843838) is also available separately.

Testing Data

Immunogen: Synthetic peptide mapping to the C-terminus of rat FasL

Background: Fas/Apo-1/CD95 is a cell surface type-I membrane glycoprotein with an apparent molecular weight of 44 kDa on SDS-PAGE. Fas is a member of the TNFR family that includes TNFR1, TNFR2, CD27, CD30, and CD40. Binding of Fas-ligand (FasL) to Fas or crosslinking of Fas by anti-Fas monoclonal antibodies leads to apoptosis in Fas expressing cells. Both Fas and TNF-R contain the 70 amino acids cytoplasmic "death domain" that is responsible for transmitting signal for apoptosis.

6753818

NP_034307.1

NM_010177.4

P41047

FasL: Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects. Homotrimer (Probable). Interacts with ARHGAP9, BAIAP2L1, BTK, CACNB3, CACNB4, CRK, DLG2, DNMBP, DOCK4, EPS8L3, FGR, FYB, FYN, HCK, ITK, ITSN2, KALRN, LYN, MACC1, MIA, MPP4, MYO15A, NCF1, NCK1, NCK2, NCKIPSD, OSTF1, PIK3R1, PSTPIP1, RIMBP3C, SAMSN1, SH3GL3, SH3PXD2B, SH3PXD2A, SH3RF2, SKAP2, SNX33, SNX9, SORBS3, SPTA1, SRC, SRGAP1, SRGAP2, SRGAP3, TEC, TJP3 and YES1. Belongs to the tumor necrosis factor family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cytokine; Membrane protein, integral; Apoptosis. Cellular Component: extracellular space; lysosome; cytoplasmic membrane-bound vesicle; integral to membrane; extracellular region; caveola; lipid raft; membrane; perinuclear region of cytoplasm; plasma membrane; cytoplasmic vesicle; nucleus; external side of plasma membrane. Molecular Function: death receptor binding; cytokine activity; tumor necrosis factor receptor binding. Biological Process: caspase activation; transcription, DNA-dependent; positive regulation of apoptosis; apoptosis; negative regulation of transcription from RNA polymerase II promoter; cellular chloride ion homeostasis; endosomal lumen acidification; inflammatory cell apoptosis; negative regulation of angiogenesis; regulation of transcription, DNA-dependent; induction of apoptosis via death domain receptors; positive regulation of neuron apoptosis; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of cell proliferation; retinal cell programmed cell death; immune response

0.03 mg

150 USD

0.1 mg

290