antibody

TrkA Antibody

MBS840182

0.03 mg

150 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, immunohistochemistry, immunoprecipitation

Antibody

TrkA Antibody

[TrkA]

[Trk-A; TRK1; NTRK1; TRK; CIPA]

[TRKA; High affinity nerve growth factor receptor; high affinity nerve growth factor receptor; neurotrophic tyrosine kinase, receptor, type 1; Neurotrophic tyrosine kinase receptor type 1; TRK1-transforming tyrosine kinase protein; Tropomyosin-related kinase A; Tyrosine kinase receptor; Tyrosine kinase receptor A; Trk-A; gp140trk; p140-TrkA]

[TrkA]

[NTRK1; NTRK1; MTC; TRK; TRK1; TRKA; Trk-A; p140-TrkA; MTC; TRK; TRKA; Trk-A]

NTRK1_HUMAN

Polyclonal

Rabbit

Human, Mouse, Rat

796

The antibody detects ~140 kDa TrkA. It does not cross-react with TrkB or TrkC.

Affinity purified

Phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% proclin.

Store at -20°C. For long-term storage, aliquot and freeze at -70°C. Avoid repeated freeze/defrost cycles.

Western Blot (WB), Immunohistochemistry (IHC) / Immunoprecipitation (IP) (predicted)

Western blotting (0.5-4 ug/ml) and in Immunohistochemistry (5 ug/mL). However, the optimal conditions should be determined individually. Blocking peptide (Cat. # MBS843988) is available separately.

Testing Data

Immunogen: Synthetic peptide surrounding amino acid 494 of human TrkA

Background: TrkA, the high affinity Nerve Growth Factor (NGF) receptor, autophosphorylates on tyrosine to activate multiple effectors. Phosphorylation at Tyr490 is required for Shc association and activation of the Ras-MAP kinase cascade. Residues Tyr674/675 lie within the catalytic domain, and phosphorylation at these sites reflects Trk kinase activity.

3869113

BAA34355

ARMS-mediated Activation Pathway (1269471); Activation Of TRKA Receptors Pathway (1269461); Apoptosis Pathway (83060); Apoptosis Pathway (470); BDNF Signaling Pathway (712093); Central Carbon Metabolism In Cancer Pathway (1059538); Central Carbon Metabolism In Cancer Pathway (1084231); Endocytosis Pathway (102279); Endocytosis Pathway (102181); Frs2-mediated Activation Pathway (1269470)

This gene encodes a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. The presence of this kinase leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in this gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation and cancer. Alternate transcriptional splice variants of this gene have been found, but only three have been characterized to date. [provided by RefSeq, Jul 2008]

TrkA: a receptor tyrosine kinase of the Trk family. High affinity receptor for nerve growth factor, neurotrophin-3 and neurotrophin-4/5 but not brain- derived neurotrophic factor. Known substrates include Shc, PI-3K, and PLC-gamma-1. Has a crucial role in the development and function of the nociceptive reception system. Two splice variant isoforms have been described. Protein type: Membrane protein, integral; Kinase, protein; Protein kinase, tyrosine (receptor); EC 2.7.10.1; Protein kinase, TK; Oncoprotein; TK group; Trk family. Chromosomal Location of Human Ortholog: 1q21-q22. Cellular Component: cell surface; protein complex; integral to plasma membrane; late endosome membrane; early endosome; dendrite; cell soma; axon; early endosome membrane; late endosome; plasma membrane; cytoplasmic vesicle; endosome; receptor complex. Molecular Function: neurotrophin p75 receptor binding; neurotrophin binding; protein binding; protein homodimerization activity; nerve growth factor receptor activity; ephrin receptor binding; nerve growth factor binding; transmembrane receptor protein tyrosine kinase activity; ATP binding. Biological Process: circadian rhythm; axon guidance; mechanoreceptor differentiation; peptidyl-tyrosine phosphorylation; nerve growth factor receptor signaling pathway; activation of MAPKK activity; protein amino acid autophosphorylation; positive regulation of synaptic transmission, glutamatergic; olfactory nerve development; protein amino acid phosphorylation; activation of NF-kappaB transcription factor; negative regulation of cell proliferation; response to radiation; sympathetic nervous system development; learning and/or memory; small GTPase mediated signal transduction; response to axon injury; negative regulation of neuron apoptosis; detection of temperature stimulus involved in sensory perception of pain; response to electrical stimulus; response to hydrostatic pressure; aging; response to drug; response to nutrient levels; phosphoinositide-mediated signaling; adenylate cyclase activation; Sertoli cell development; detection of mechanical stimulus involved in sensory perception of pain; positive regulation of angiogenesis; positive regulation of programmed cell death; response to ethanol; phospholipase C activation; B cell differentiation; Ras protein signal transduction; positive regulation of Ras protein signal transduction; response to activity; transmembrane receptor protein tyrosine kinase signaling pathway. Disease: Thyroid Carcinoma, Familial Medullary; Insensitivity To Pain, Congenital, With Anhidrosis

0.03 mg

150 USD

0.1 mg

290