L-Selectin / CD62L Antibody | MyBioSource MBS840167 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

L-Selectin / CD62L Antibody

catalog :

MBS840167

quantity :

0.03 mg

price :

150 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of L-Selectin expression in Jurkat cell lysate.

product information

catalog number :

MBS840167

products type :

Antibody

products full name :

L-Selectin / CD62L Antibody

products short name :

[L-Selectin/CD62L]

products name syn :

[SELL; Lymphocyte adhesion molecule 1]

other names :

[L-selectin; L-selectin; L-selectin; selectin L; CD62 antigen-like family member L; Leukocyte adhesion molecule 1; LAM-1; Leukocyte surface antigen Leu-8; Leukocyte-endothelial cell adhesion molecule 1; LECAM1; Lymph node homing receptor; TQ1; gp90-MEL; CD_antigen: CD62L]

other gene names :

[SELL; SELL; TQ1; LAM1; LEU8; LNHR; LSEL; CD62L; LYAM1; PLNHR; LECAM1; LNHR; LYAM1; LAM-1; LECAM1]

uniprot entry name :

LYAM1_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human

sequence length :

385

specificity :

The antibody recognizes L-Selectin in human sample, and in a lesser extent to mouse, and rat samples.

purity :

Affinity purified

form :

100 ug (0.5mg/ml) protein A purified rabbit anti-L-Selectin polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal. Appearance: Colorless liquid

concentration :

0.5 mg/ml

storage stability :

Store at -20°C. For long term storage, aliquot and freeze at –70°C. Avoid repeated freeze/defrost cycles.

tested application :

Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC)

app notes :

The antibody can be used for Western blotting (0.5-4 ug/ml). Based on researcher’s feedback, it can also be used in immunoprecipitation (10-20 ug/ml) and immunohistochemistry (10-20 ug/ml). However, the optimal concentrations should be determined individually.

image1 heading :

Testing Data

other info1 :

Immunogen: Synthetic peptide surrounding amino acid 69 of human L-Selectin

other info2 :

Handling: The antibody solution should be gently mixed before use.

products description :

Background: Selectins are a family of carbohydrate-binding proteins involved in mediating cellular interactions with leukocytes. E-Selectin (also called ELMA-1) expressed on endothelial cells. L-Selectin (also called LECAM-1) is expressed on the majority of B and naïve T cells and on most monocytes, neurophils and eosinophils. P-Selectin (also called GMP-140) expressed on activated platelets and endothelial cells. Selectins interact with specific carbohydrates expressed by activated endothelial cells, monocytes, and neutrophils.

ncbi gi num :

262206315

ncbi acc num :

NP_000646.2

ncbi gb acc num :

NM_000655.4

uniprot acc num :

P14151

ncbi pathways :

Adaptive Immune System Pathway (1269171); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cell Surface Interactions At The Vascular Wall Pathway (1269373); Hemostasis Pathway (1269340); Immune System Pathway (1269170); Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway (1269201)

ncbi summary :

This gene encodes a cell surface adhesion molecule that belongs to a family of adhesion/homing receptors. The encoded protein contains a C-type lectin-like domain, a calcium-binding epidermal growth factor-like domain, and two short complement-like repeats. The gene product is required for binding and subsequent rolling of leucocytes on endothelial cells, facilitating their migration into secondary lymphoid organs and inflammation sites. Single-nucleotide polymorphisms in this gene have been associated with various diseases including immunoglobulin A nephropathy. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2009]

uniprot summary :

SELL: Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia. Belongs to the selectin/LECAM family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 1q23-q25. Cellular Component: integral to plasma membrane; plasma membrane; external side of plasma membrane. Molecular Function: heparin binding; protein binding; protease binding; cell adhesion molecule binding; glycosphingolipid binding; carbohydrate binding. Biological Process: regulation of immune response; cell adhesion; blood coagulation; leukocyte migration; response to ATP. Disease: Iga Nephropathy, Susceptibility To, 1

size1 :

0.03 mg

price1 :

150 USD

size2 :

0.1 mg

price2 :

300

more info or order :

MyBioSource product webpage