Bcl-2 Antibody (Clone Bcl-2 / 100) | MyBioSource MBS840129 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Bcl-2 Antibody (Clone Bcl-2 / 100)

catalog :

MBS840129

quantity :

0.1 mg

price :

335 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[Bcl-2/100]

reactivity :

human

application :

western blot, immunoprecipitation

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS840129

products type :

Antibody

products full name :

Bcl-2 Antibody (Clone Bcl-2 / 100)

products short name :

[Bcl-2]

products name syn :

[BCL2; BCL 2]

other names :

[Human B-cell leukemia/lymphoma 2 (bcl-2) proto-oncogene mRNA encoding bcl-2-alpha protein, complete cds; apoptosis regulator Bcl-2; B-cell CLL/lymphoma 2]

products gene name :

[Bcl-2]

other gene names :

[BCL2; Bcl-2; PPP1R50]

clonality :

Monoclonal

isotype :

IgG 1

clone :

[Bcl-2/100]

host :

Mouse

reactivity :

Human. Others are not tested.

sequence length :

5086

specificity :

Recognize the 26 kDa human Bcl-2

purity :

Purity was >95%.

form :

100 ug (0.5 mg/ml) protein A purified from (low FBS containing) tissue culture supernatant. The antibody was formulated in PBS containing 1 mg/ml BSA and 1.5 mM sodium azide and 50% glycerol

storage stability :

Store at -20°C . For long term storage, aliquot and refreeze at -70°C. Avoid repeated freeze/ defrost cycles.

tested application :

Western Blot, Immunoprecipitation, Immunohistochemistry and Flow cytometry. However the optimal conditions should be determined individually.

app notes :

Western blot (1-4 ug/ml).

other info1 :

Immunogen: Synthetic peptide corresponding to resides surrounding a.a. 44 of Human Bcl-2

other info2 :

Handling: The antibody solution should be gently mixed before use.

products description :

Background: Bcl-2 (B-cell leukemia/lymphoma 2) gene was discovered by walking the chromosomal breakpoints t(14;18). This gene consists of two exons and several transcripts that encode two different protein products, Bcl-2alpha and Bcl-2beta. Both proteins are approximately 26-30 kDa and differ at their C-terminal region. Bcl-2 suppresses apoptosis (programmed cell death) and this property appears to be modulated by homodimerization or association with Bax and Bad, two cell death promoters

ncbi gi num :

179366

ncbi acc num :

AAA51813.1

uniprot acc num :

M13994

ncbi pathways :

ATF-2 Transcription Factor Network Pathway (138006); Activation Of BAD And Translocation To Mitochondria Pathway (1270271); Activation Of BH3-only Proteins Pathway (1270270); Adrenergic Signaling In Cardiomyocytes Pathway (908257); Adrenergic Signaling In Cardiomyocytes Pathway (909696); Amyotrophic Lateral Sclerosis (ALS) Pathway (920975); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Apoptosis Pathway (198797); Apoptosis Pathway (83060)

ncbi summary :

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq, Jul 2008]

uniprot summary :

Bcl-2: a antiapoptotic member of the Bcl-2 family. Regulates cell death by controlling the mitochondrial membrane permeability. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). Phosphorylation by JNKs may increase its antiapoptotic functions. Protein type: Membrane protein, integral; Apoptosis; Autophagy; Oncoprotein. Chromosomal Location of Human Ortholog: 18q21.3. Cellular Component: pore complex; mitochondrial outer membrane; endoplasmic reticulum membrane; nuclear membrane; membrane; mitochondrion; endoplasmic reticulum; cytoplasm; nucleus; cytosol; myelin sheath. Molecular Function: identical protein binding; protein binding; protein homodimerization activity; protease binding; protein phosphatase 2A binding; sequence-specific DNA binding; protein heterodimerization activity; channel activity; ubiquitin protein ligase binding; BH3 domain binding; channel inhibitor activity; transcription factor binding. Biological Process: focal adhesion formation; positive regulation of catalytic activity; response to nicotine; developmental growth; renal system process; pigment granule organization and biogenesis; protein polyubiquitination; response to toxin; response to glucocorticoid stimulus; T cell differentiation in the thymus; ear development; lymphoid progenitor cell differentiation; positive regulation of multicellular organism growth; female pregnancy; glomerulus development; negative regulation of mitochondrial depolarization; post-embryonic development; cochlear nucleus development; cellular response to glucose starvation; negative regulation of myeloid cell apoptosis; B cell receptor signaling pathway; regulation of mitochondrial membrane potential; negative regulation of ossification; positive regulation of B cell proliferation; regulation of transmembrane transporter activity; T cell homeostasis; negative regulation of neuron apoptosis; cell growth; defense response to virus; spleen development; response to drug; positive regulation of neuron maturation; release of cytochrome c from mitochondria; regulation of protein homodimerization activity; axon regeneration; cell aging; actin filament organization; digestive tract morphogenesis; regulation of calcium ion transport; positive regulation of cell growth; organ growth; DNA damage response, signal transduction resulting in induction of apoptosis; induction of apoptosis via death domain receptors; gland morphogenesis; negative regulation of osteoblast proliferation; regulation of mitochondrial membrane permeability; regulation of nitrogen utilization; metanephros development; oocyte development; negative regulation of apoptosis; B cell proliferation; negative regulation of autophagy; regulation of protein heterodimerization activity; behavioral fear response; melanin metabolic process; regulation of cell-matrix adhesion; negative regulation of retinal cell programmed cell death; apoptosis; regulation of protein stability; protein amino acid dephosphorylation; positive regulation of smooth muscle cell migration; response to radiation; B cell homeostasis; ovarian follicle development; positive regulation of skeletal muscle fiber development; positive regulation of melanocyte differentiation; melanocyte differentiation; response to gamma radiation; regulation of viral genome replication; transmembrane transport; response to iron ion; negative regulation of cellular pH reduction; negative regulation of cell migration; mesenchymal cell development; ossification; hair follicle morphogenesis; CD8-positive, alpha-beta T cell lineage commitment; thymus development; B cell lineage commitment; male gonad development; peptidyl-threonine phosphorylation; positive regulation of peptidyl-serine phosphorylation; humoral immune response; response to UV-B; endoplasmic reticulum calcium ion homeostasis; neuron apoptosis; peptidyl-serine phosphorylation; axonogenesis; response to hydrogen peroxide; homeostasis of number of cells within a tissue; ureteric bud branching; response to cytokine stimulus; innate immune response; negative regulation of cell growth; response to DNA damage stimulus; induction of apoptosis by oxidative stress

size1 :

0.1 mg

price1 :

335 USD

more info or order :

MyBioSource product webpage