catalog number :
MBS835324
products full name :
AMPK alpha antibody
products short name :
AMPK alpha
products name syn :
Polyclonal AMPK alpha; Anti-AMPK alpha; 5-AMP-activated protein kinase anibody; Ab 174/172
products gene name :
AMPK alpha
reactivity :
Human, Mouse, Rat
purity :
AMPK alpha antibody was purified by immunoaffinity chromatography.
form :
Supplied in liquid form in PBS buffer (no MG2+ or Ca2+), pH 7.4, 150 mm NaCl, 0.02% sodium azide and 50% glycerol.
storage stability :
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
tested application :
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
app notes :
IF: 1:100-1:200. IHC: 1:50-1:100. WB: 1:500-1:1000
other info1 :
Biological Significance: AMPK, a member of the SNF1 (sucrose nonfermentor) kinase family AMPK is a heterotrimeric protein comprising alpha (63 kDa), beta (38 kDa) and gamma (38 kDa) subunits. The alpha subunit is the catalytic subunit (which requires phosphorylation to be active) (2-3). Beta and gamma are noncatalytic subunits, although they have been found to interact with the active subunit in liver. AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase.
other info2 :
Immunogen: AMPK alpha antibody was raised in Rabbit using the peptide sequence around aa 172~176/170~174 (L-R-T-S-C) derived from Human AMPKA1/AMPKA2 as the immunogen.
products categories :
Proteases, Inhibitors, & Enzymes
products description :
Rabbit polyclonal AMPK alpha antibody