catalog number :
MBS834371
products full name :
Ly6A/E antibody (PE)
products short name :
[Ly6A/E]
products name syn :
[Monoclonal Ly6A/E; Anti-Ly6A/E; Ly6AE; Ly6E; LyA/E 6; LyA/E-6; Ly6A/E; Ly6A; Sca1]
products gene name :
[Ly6A/E]
form :
Affinity purified liquid in PBS, pH 7.2, with 0.1% Gelatin, and 0.09% NaN3
concentration :
0.2 mg/ml
storage stability :
Store 4°C in the dark. DO NOT FREEZE!
tested application :
Flow Cytometry (FC/FACS)
app notes :
FC:0.5 ug/test. A test is defined as the amount (ug)/test of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
image1 heading :
Flow Cytometry (FC/FACS)
other info1 :
Biological Significance: The D7 monoclonal antibody reacts with mouse Sca-1, an 18 kDa member of the Ly-6 family of GPI-linked surface proteins. D7 reacts with both Ly-6E.1 and Ly-6A.2 molecules expressed by mouse hematopoietic stem cells, myeloid population and peripheral T and B cells. Non-activated splenocytes from Ly-6.2 strains including C57BL, SJL, 129, AKR, and others demonstrate higher frequency of D7-positive cells compared to the Ly-6.1 strains such as BALB/c, C3H, NZB and others. Sca-1 is expressed at high levels upon activation regardless of the Ly-6 haplotype. The Ly-6 family is involved in regulation and function of T cell activation. Sca-1 is a major phenotypic marker for mouse hematopoietic progenitor/stem cell subset.
other info2 :
Tag/Conjugate: PE
products categories :
Immunology; Conjugated Monoclonal Antibodies
products description :
Rat monoclonal Ly6A/E antibody (PE)