catalog number :
MBS833304
products full name :
YFP antibody
products short name :
[YFP]
products name syn :
[Monoclonal YFP antibody, Anti-YFP antibody, Yellow Fluorescent Protein antibody, Yellow Fluorescent Protein antibody, YFP antibody, Anti YFP antibody]
products gene name :
[YFP]
reactivity :
Recognizes native and denatured forms of GFP and its variants EGFP, YFP, EYFP, and CFP.
purity :
YFP antibody was purified by affinity chromatography
form :
Supplied in 10mM PBS, pH7.2 with 0.1% sodium azide
storage stability :
Shipping: Blue ice. Upon receipt store at -20°C.
tested application :
Suitable for Western Blot (WB), ELISA (EIA), Dot Blot, Immunostaining.
app notes :
Western Blot: 1:1000. Use an anti-Mouse secondary antibody to detect the antibody.
other info1 :
Biological Significance: Yellow Fluorescent Protein (YFP) is a genetic mutant of green fluorescent protein, derived from Aequorea victoria. Its excitation peak is 514nm and its emission peak is 527nm. Like green fluorescent protein (GFP), it is a useful tool in cell and molecular biology, usually explored using fluorescence microscopy. Three improved versions of YFP are Citrine, Venus, and Ypet. They have reduced chloride sensitivity, faster maturation, and increased brightness (product of the extinction coefficient and quantum yield). Typically, yellow FPs serve as the acceptor for genetically-encoded FRET sensors of which the most likely donor FP is mCFP (monomeric cyan FP). The red-shift relative to GFP is caused by a Pi-Pi stacking interaction as a result of the T203Y mutation, which essentially increases the polarizability of the local chromophore environment as well as providing additional electron density into the chromophore.
other info2 :
Immunogen: GFP from the jellyfish aequorea Victoria N-terminal peptide-KLH conjugates
products description :
Monoclonal YFP, Anti-YFP, Yellow Fluorescent Protein, YFP antibody, Anti YFP
ncbi acc num :
AFI26426.1