product summary
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company name :
MyBioSource
product type :
antibody
product name :
CD244.2 antibody
catalog :
MBS832390
quantity :
0.05 mg
price :
185 USD
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
244F4
application :
western blot, immunohistochemistry, immunoprecipitation, flow cytometry, FACS
more info or order :
product information
catalog number :
MBS832390
products type :
Antibody
products full name :
CD244.2 antibody
products short name :
CD244.2
products name syn :
Monoclonal CD244.2; Anti-CD244.2; B6 Alloantigen; CD-244.2; CD 244.2; CD244.2
products gene name :
CD244.2
clonality :
Monoclonal
isotype :
IgG2a
clone :
244F4
host :
Rat
purity :
CD244.2 antibody was purified by affinity chromatography.
form :
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
concentration :
500 ug/ml
storage stability :
Store at 2-8 degree C. Do not freeze. Protect from light.
tested application :
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Functional Assay, Immunoprecipitation (IP), Western Blot (WB)
app notes :
FC: <= 0.125 ug/test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest
other info1 :
Biological Significance: Mouse CD244, also known as 2B4, is a receptor belonging to the CD2 family of proteins involved in non-MHC dependent cytotoxicity. Expression is found on natural killer cells and many NKT cells and CD8+ cells. In C57BL/6 mice, two splice variants exist that differ in the length of the cytoplasmic domain. The long form functions in an inhibitory manner through signaling through SHP-2, while the short form functions in an activating manner. The ligand for CD244 is CD48 which is expressed on all hematopoietic cells. Recent data suggests that CD244 interactions with CD48, but not CD2 interaction with CD48, is essential for IL-2?driven expansion and activation of murine NK cells.Reports show CD244 expression can discriminate hematopoietic progenitors. Hematopoietic stem cells are highly purified as CD150+CD244-CD48- cells while non-self-renewing multipotent hematopoietic progenitors are CD244+CD150-CD48- and the most restricted progenitors are CD48+CD244+CD150-.244F4 was generated against cell lines expressing CD244. It reacts with the C57BL/6 but not Balb/c due to polymorphisms present in the immunoglobulin V domain of the protein. The epitope recognized by 244F4 is different from that of 2B4 therefore these antibodies do not cross-block the binding to the respective epitopes.
products categories :
Immunology
products description :
Rat monoclonal CD244.2 antibody
size1 :
0.05 mg
price1 :
185 USD
size2 :
0.1 mg
price2 :
250
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
"MyBioSource offers the best products at unbeatable prices."
Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.
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