antibody

CD160 antibody (PE)

MBS832170

0.05 mg

250 USD

monoclonal

rat

PE

CNX46-3

flow cytometry, FACS

Antibody

CD160 antibody (PE)

CD160

Monoclonal CD160; Anti-CD160; NK1; CD-160; CD 160; CD160; NK28; BY55

CD160 antigen; CD160 antigen; CD160 antigen; CD160 molecule; Natural killer cell receptor BY55; CD_antigen: CD160

CD160

CD160; CD160; NK1; BY55; NK28; BY55

BY55_HUMAN

Monoclonal

IgG2a kappa

CNX46-3

Rat

181

Human

Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.

200 ug/ml

Store at 2-8 degree C. Do not freeze. Protect from light.

Flow Cytometry (FC/FACS)

FC: <= 0.125 ug/test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest

Biological Significance: CD160 is a glycosylphosphatidylinositol -anchored Ig-like glycoprotein first identified on human lymphocytes with the monoclonal antibody BY55. In mice, CD160 is expressed on almost all iIELs, NKT cells, most TCRgamma delta T cells, few NK cells and a minor subset of CD8+ T cells. Murine CD160 has been shown to bind to a wide range of classical and non classical MHC class I molecules and regulate NK cell activation. In vitro, CD3 activation of murine CD8+ T cells increases the expression of CD160 and induces the release of soluble CD160. In human, CD160 mAb cross-linking triggers TNF alpha, IFNgamma and IL-6 cytokine production by peripheral blood NK cells and inhibits tube formation and induces apoptosis of endothelial cells. In mice, cross-linking of CD160 with the CNX46-3 antibody regulates NK cell activation both positively and negatively, depending on the stimulus.

Tag/Conjugate: PE

Immunology; Conjugated Monoclonal Antibodies

Rat monoclonal CD160 antibody (PE)

5901910

NP_008984.1

NM_007053.3

O95971

19,810 Da

Adaptive Immune System Pathway (366160); Immune System Pathway (106386); Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway (106413)

CD160 is an 27 kDa glycoprotein which was initially identified with the monoclonal antibody BY55. Its expression is tightly associated with peripheral blood NK cells and CD8 T lymphocytes with cytolytic effector activity. The cDNA sequence of CD160 predicts a cysteine-rich, glycosylphosphatidylinositol-anchored protein of 181 amino acids with a single Ig-like domain weakly homologous to KIR2DL4 molecule. CD160 is expressed at the cell surface as a tightly disulfide-linked multimer. RNA blot analysis revealed CD160 mRNAs of 1.5 and 1.6 kb whose expression was highly restricted to circulating NK and T cells, spleen and small intestine. Within NK cells CD160 is expressed by CD56dimCD16+ cells whereas among circulating T cells its expression is mainly restricted to TCRgd bearing cells and to TCRab+CD8brightCD95+CD56+CD28-CD27-cells. In tissues, CD160 is expressed on all intestinal intraepithelial lymphocytes. CD160 shows a broad specificity for binding to both classical and nonclassical MHC class I molecules. [provided by RefSeq, Jul 2008]

0.05 mg

250 USD

0.1 mg

395