HAND1 antibody | MyBioSource MBS831724 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

HAND1 antibody

catalog :

MBS831724

quantity :

0.1 mL

price :

760 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[1G10]

reactivity :

human

application :

western blot, immunocytochemistry, flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant HAND1 protein (Right) or empty vector (Left) detected with HAND1 antibody

image 2 :

Immunofluorescent staining of COS7 cells transiently transfected with recombinant HAND1 protein using HAND1 antibody

image 3 :

Flow Cytometric analysis of Hela cells using HAND1 antibody (Red), compared to a nonspecific negative control antibody (Blue).

product information

catalog number :

MBS831724

products type :

Antibody

products full name :

HAND1 antibody

products short name :

[HAND1]

products name syn :

[Monoclonal HAND1; Anti-HAND1; Heart and neural crest derivatives expressed 1; bHLHa27; Hxt; Thing1; eHand; Class A basic helix-loop-helix protein 27; HAND1; HAND 1; HAND-1]

other names :

[heart- and neural crest derivatives-expressed protein 1; Heart- and neural crest derivatives-expressed protein 1; heart- and neural crest derivatives-expressed protein 1; heart and neural crest derivatives expressed 1; Class A basic helix-loop-helix protein 27; bHLHa27; Extraembryonic tissues, heart, autonomic nervous system and neural crest derivatives-expressed protein 1; eHAND]

products gene name :

[HAND1]

other gene names :

[HAND1; HAND1; Hxt; eHand; Thing1; bHLHa27; BHLHA27; EHAND; bHLHa27; eHAND]

uniprot entry name :

HAND1_HUMAN

clonality :

Monoclonal

isotype :

IgG2b

clone :

[1G10]

host :

Mouse

reactivity :

Human

purity :

HAND1 antibody was purified by affinity chromatography.

form :

Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.

concentration :

1.0mg/ml

storage stability :

Shipping: Blue Ice. Upon receipt, store at -20°C. Avoid repeat freeze-thaw cycles.

tested application :

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB)

app notes :

IF: 1:100. FC: 1:100. WB: 1:500

image1 heading :

Western Blot (WB)

image2 heading :

Immunofluorescence (IF)

image3 heading :

Flow Cytometry (FC/FACS)

other info2 :

Immunogen: HAND1 antibody was raised in mouse using a full length recombinant protein of human HAND1 (NP_004812) produced in E Coli as the immunogen.

products categories :

DNA & RNA

products description :

HAND1 belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementaryfashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediatorsof congenital heart disease. In addition, it has been suggested that this transcription factor may be required forearly trophoblast differentiation.

ncbi gi num :

4758506

ncbi acc num :

NP_004812

ncbi gb acc num :

NM_004821.2

uniprot acc num :

O96004

ncbi mol weight :

23,627 Da

ncbi pathways :

Heart Development Pathway (198802); Neural Crest Differentiation Pathway (672460); Signaling Pathways Regulating Pluripotency Of Stem Cells (1026136); Signaling Pathways Regulating Pluripotency Of Stem Cells (1033502)

ncbi summary :

The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, it has been suggested that this transcription factor may be required for early trophoblast differentiation. [provided by RefSeq, Jul 2008]

uniprot summary :

HAND1: Transcription factor that plays an essential role in both trophoblast-giant cells differentiation and in cardiac morphogenesis. In the adult, could be required for ongoing expression of cardiac-specific genes. Binds the DNA sequence 5 - NRTCTG-3 (non-canonical E-box). Protein type: Cell development/differentiation; DNA-binding; Transcription factor; Nucleolus. Chromosomal Location of Human Ortholog: 5q33. Cellular Component: nucleoplasm; cytoplasm; nucleolus; nucleus. Molecular Function: identical protein binding; protein binding; protein homodimerization activity; enzyme binding; protein heterodimerization activity; transcription coactivator activity; bHLH transcription factor binding; transcription corepressor activity; transcription factor binding. Biological Process: transcription from RNA polymerase II promoter; blastocyst development; mesoderm formation; negative regulation of transcription factor activity; heart development; embryonic heart tube development; ventricular cardiac muscle morphogenesis; positive regulation of transcription from RNA polymerase II promoter; heart looping; negative regulation of transcription from RNA polymerase II promoter; angiogenesis; negative regulation of transcription, DNA-dependent; odontogenesis of dentine-containing teeth; trophectodermal cell differentiation

size1 :

0.1 mL

price1 :

760 USD

more info or order :

MyBioSource product webpage