antibody

Mucin antibody

MBS830515

1 mL

550 USD

monoclonal

mouse

nonconjugated

45 M1

immunohistochemistry, immunocytochemistry

Antibody

Mucin antibody

Mucin

Monoclonal Mucin; Anti Mucin

mucin, partial; Mucin; mucin-4; mucin 4, cell surface associated; Mucin

MUC4; MUC4; ASGP; MUC-4; HSA276359

O76037_HUMAN

Monoclonal

IgG1

45 M1

Mouse

Human, most mammals (including monkey, cat, pig, rat, mouse, rabbit, hedgehog), chicken. Negative to cow.

1045

Culture supernatant with 0.09% NaN3. Diluttion Buffer: PBS with 1% BSA and 0.1% NaN3

Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Immunofluorescence (IF), Immunohistochemistry (IHC)

IHC: 1:10 - 1:40

Biological Significance: Mucins are a family of high molecular weight, heavily glycosylated proteins (glycoconjugates) produced by epithelial tissues in most metazoans. Mucins' key characteristic is their ability to form gels; therefore they are a key component in most gel-like secretions, serving functions from lubrication to cell signalling to forming chemical barriers. They often take an inhibitory role.

Immunogen: Mucin antibody was raised in mouse using Mucin isolated from ovarian mucinous cysts and colonic mucosa as the immunogen.

Immunology

Mouse monoclonal Mucin antibody

3649741

CAA03985.1

O76037

106,738 Da

Metabolism Of Proteins Pathway (106230); O-linked Glycosylation Pathway (1127679); O-linked Glycosylation Of Mucins Pathway (530747); Post-translational Protein Modification Pathway (161012); Termination Of O-glycan Biosynthesis Pathway (530748)

The major constituents of mucus, the viscous secretion that covers epithelial surfaces such as those in the trachea, colon, and cervix, are highly glycosylated proteins called mucins. These glycoproteins play important roles in the protection of the epithelial cells and have been implicated in epithelial renewal and differentiation. This gene encodes an integral membrane glycoprotein found on the cell surface, although secreted isoforms may exist. At least two dozen transcript variants of this gene have been found, although for many of them the full-length transcript has not been determined or they are found only in tumor tissues. This gene contains a region in the coding sequence which has a variable number ( 100) of 48 nt tandem repeats. [provided by RefSeq, Jul 2008]

MUC4: May play a role in tumor progression. Ability to promote tumor growth may be mainly due to repression of apoptosis as opposed to proliferation. Has anti-adhesive properties. Seems to alter cellular behavior through both anti-adhesive effects on cell-cell and cell-extracellular matrix interactions and in its ability to act as an intramembrane ligand for ERBB2. Plays an important role in cell proliferation and differentiation of epithelial cells by inducing specific phosphorylation of ERBB2. The MUC4-ERBB2 complex causes site-specific phosphorylation of the ERBB2 Tyr-1248 . In polarized epithelilal cells segragates ERBB2 and other ERBB receptors and prevents ERBB2 from acting as a coreceptor. The interaction with ERBB2 leads to enhanced expression of CDKN1B. The formation of a MUC4-ERBB2-ERBB3-NRG1 complex leads to down-regulation of CDKN1B, resulting in repression of apoptosis and stimulation of proliferation. 14 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 3q29. Cellular Component: extracellular space; proteinaceous extracellular matrix; membrane; Golgi lumen; integral to plasma membrane; vesicle. Molecular Function: ErbB-2 class receptor binding; extracellular matrix constituent, lubricant activity. Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; maintenance of gastrointestinal epithelium; cell-matrix adhesion; post-translational protein modification

1 mL

550 USD