Anti-Glucocorticoid Receptor (pS226) Antibody | MyBioSource MBS8304177 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Glucocorticoid Receptor (pS226) Antibody

catalog :

MBS8304177

quantity :

0.03 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of Glucocorticoid Receptor (pS226) expression in A549 (A), Hela (B), U87MG (C), PC12 (D) whole cell lysates.

image 2 :

Immunohistochemical analysis of Glucocorticoid Receptor (pS226) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

product information

catalog number :

MBS8304177

products type :

Antibody

products full name :

Anti-Glucocorticoid Receptor (pS226) Antibody

products short name :

[Glucocorticoid Receptor]

products name syn :

[GRL; Glucocorticoid receptor; GR; Nuclear receptor subfamily 3 group C member 1]

other names :

[glucocorticoid receptor isoform alpha; Glucocorticoid receptor; glucocorticoid receptor; nuclear receptor subfamily 3 group C member 1; Nuclear receptor subfamily 3 group C member 1]

products gene name :

[NR3C1]

other gene names :

[NR3C1; NR3C1; GR; GCR; GRL; GCCR; GCRST; GRL; GR]

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse, Rat, Rabbit, Sheep

sequence length :

777

specificity :

Recognizes endogenous levels of Glucocorticoid Receptor (pS226) protein.

purity :

Purified by immunogen affinity chromatography.

form :

Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.

storage stability :

Shipped at 4°C. Upon delivery aliquot and store at-20 degree C for one year. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC)

app notes :

WB: 1/500-1/1000. IHC: 1/50-1/100

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

other info1 :

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Glucocorticoid Receptor. The exact sequence is proprietary.

products description :

Rabbit polyclonal antibody to Glucocorticoid Receptor (pS226)

ncbi gi num :

4504133

ncbi acc num :

NP_000167.1

ncbi gb acc num :

NM_000176.2

uniprot acc num :

P04150

ncbi pathways :

Adipogenesis Pathway (198832); BMAL1:CLOCK/NPAS2 Activates Circadian Expression Pathway (477138); Circadian Clock Pathway (187173); FOXA2 And FOXA3 Transcription Factor Networks Pathway (137911); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); Glucocorticoid Receptor Regulatory Network Pathway (138014); Neuroactive Ligand-receptor Interaction Pathway (83053); Neuroactive Ligand-receptor Interaction Pathway (462); Nuclear Receptor Transcription Pathway (105979)

ncbi summary :

This gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in this gene are associated with generalized glucocorticoid resistance. Alternative splicing of this gene results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described, and shown to be functional, displaying diverse cytoplasm-to-nucleus trafficking patterns and distinct transcriptional activities (PMID:15866175). [provided by RefSeq, Feb 2011]

uniprot summary :

Receptor for glucocorticoids (GC) (PubMed:27120390). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Involved in chromatin remodeling (PubMed:9590696). Plays a role in rapid mRNA degradation by binding to the 5' UTR of target mRNAs and interacting with PNRC2 in a ligand-dependent manner which recruits the RNA helicase UPF1 and the mRNA-decapping enzyme DCP1A, leading to RNA decay (PubMed:25775514). Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth ().

size1 :

0.03 mL

price1 :

165 USD

size2 :

0.1 mL

price2 :

245

size3 :

0.2 mL

price3 :

350

more info or order :

MyBioSource product webpage