Anti-cGAS Antibody | MyBioSource MBS8292549 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-cGAS Antibody

catalog :

MBS8292549

quantity :

0.05 mL

price :

175 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of cGAS expression in Jurkat (A), mouse spleen (B) whole cell lysates.

product information

catalog number :

MBS8292549

products type :

Antibody

products full name :

Anti-cGAS Antibody

products short name :

[cGAS]

products name syn :

[C6orf150; Cyclic GMP-AMP synthase; cGAMP synthase; cGAS; h-cGAS; Mab-21 domain-containing protein 1]

other names :

[cyclic GMP-AMP synthase; Cyclic GMP-AMP synthase; cyclic GMP-AMP synthase; cyclic GMP-AMP synthase; 2'3'-cGAMP synthase]

products gene name :

[cGAS]

products gene name syn :

[MB21D1]

other gene names :

[CGAS; MB21D1; MB21D1; h-cGAS; C6orf150; h-cGAS]

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse

sequence length :

447

specificity :

Recognizes endogenous levels of cGAS protein.

purity :

The antibody was purified by immunogen affinity chromatography

form :

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

storage stability :

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC)

app notes :

WB: 1/500 - 1/2000. IHC: 1/50 - 1/200

image1 heading :

Western Blot (WB)

other info1 :

Immunogen: KLH-conjugated synthetic peptide of human cGAS

products description :

Rabbit polyclonal antibody to cGAS

ncbi gi num :

115511030

ncbi acc num :

NP_612450.2

ncbi gb acc num :

NM_138441.2

uniprot acc num :

Q8N884

ncbi pathways :

Cytosolic DNA-sensing Pathway (125137); Cytosolic DNA-sensing Pathway (124833); Cytosolic Sensors Of Pathogen-associated DNA Pathway (1269268); Immune System Pathway (1269170); Innate Immune System Pathway (1269203); STING Mediated Induction Of Host Immune Responses Pathway (1269272)

uniprot summary :

Nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP and GTP (PubMed:23258413, PubMed:23707061, PubMed:23722159, PubMed:24077100, PubMed:25131990). Catalysis involves both the formation of a 2',5' phosphodiester linkage at the GpA step and the formation of a 3',5' phosphodiester linkage at the ApG step, producing c[G(2',5')pA(3',5')p] (PubMed:28363908, PubMed:28214358). Acts as a key cytosolic DNA sensor, the presence of double-stranded DNA (dsDNA) in the cytoplasm being a danger signal that triggers the immune responses (PubMed:28363908). Binds cytosolic DNA directly, leading to activation and synthesis of cGAMP, a second messenger that binds to and activates TMEM173/STING, thereby triggering type-I interferon production (PubMed:28363908, PubMed:28314590). Has antiviral activity by sensing the presence of dsDNA from DNA viruses in the cytoplasm (PubMed:28363908). Also acts as an innate immune sensor of infection by retroviruses, such as HIV-1, by detecting the presence of reverse-transcribed DNA in the cytosol (PubMed:23929945). Detection of retroviral reverse-transcribed DNA in the cytosol may be indirect and be mediated via interaction with PQBP1, which directly binds reverse-transcribed retroviral DNA (PubMed:26046437). Also detects the presence of DNA from bacteria, such as M.tuberculosis (PubMed:26048138). cGAMP can be transferred from producing cells to neighboring cells through gap junctions, leading to promote TMEM173/STING activation and convey immune response to connecting cells (PubMed:24077100). cGAMP can also be transferred between cells by virtue of packaging within viral particles contributing to IFN-induction in newly infected cells in a cGAS-independent but TMEM173/STING-dependent manner (PubMed:26229115). In addition to antiviral activity, also involved in the response to cellular stresses, such as senescence, DNA damage or genome instability (PubMed:28738408, PubMed:28759889). Acts as a regulator of cellular senescence by binding to cytosolic chromatin fragments that are present in senescent cells, leading to trigger type-I interferon production via TMEM173/STING and promote cellular senescence (). Also involved in the inflammatory response to genome instability and double-stranded DNA breaks: acts by localizing to micronuclei arising from genome instability (PubMed:28738408, PubMed:28759889). Micronuclei, which as frequently found in cancer cells, consist of chromatin surrounded by its own nuclear membrane: following breakdown of the micronuclear envelope, a process associated with chromothripsis, MB21D1/cGAS binds self-DNA exposed to the cytosol, leading to cGAMP synthesis and subsequent activation of TMEM173/STING and type-I interferon production (PubMed:28738408, PubMed:28759889).

size1 :

0.05 mL

price1 :

175 USD

size2 :

0.1 mL

price2 :

220

size3 :

0.2 mL

price3 :

295

more info or order :

MyBioSource product webpage