ELISA/assay

Human A2M ELISA Kit

MBS825043

96 Tests

470 USD

ELISA Kit

Human A2M ELISA Kit

A2M

CPAMD5; Alpha-2-macroglobulin; Alpha-2-M; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5

alpha-2-macroglobulin; Alpha-2-macroglobulin; alpha-2-macroglobulin; alpha-2-M; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5; alpha-2-macroglobulin; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5

A2M

A2M; A2M; A2MD; CPAMD5; FWP007; S863-7; CPAMD5; Alpha-2-M

A2MG_HUMAN

Human

1474

The Human A2M ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human A2M proteins within the range of 625 pg/ml - 40000 pg/ml.

Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Sandwich ELISA (SE)

Samples: Cell Culture Supernatants, Serum, Plasma. Sensitivity: 20 pg/ml.

Principle of the Assay The Human A2M ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human A2M in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human A2M coated on a 96-well plate. Standards and samples are pipetted into the wells and A2M present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human A2M antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of A2M bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background/Introduction: Alpha-2-macroglobulin, also known as A2M or CPAMD5 is a large plasma protein found in the blood. This gene is mapped to 12p13.31. Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. This gene is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

66932947

NP_000005.2

NM_000014.4

P01023

163,291 Da

Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Degradation Of The Extracellular Matrix Pathway (576263); Extracellular Matrix Organization Pathway (576262); Formation Of Fibrin Clot (Clotting Cascade) Pathway (106057); HDL-mediated Lipid Transport Pathway (106158); Hemostasis Pathway (106028); IL6-mediated Signaling Events Pathway (137932); Intrinsic Pathway (106059)

Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. [provided by RefSeq, Jul 2008]

A2M: Is able to inhibit all four classes of proteinases by a unique trapping mechanism. This protein has a peptide stretch, called the bait region which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. Belongs to the protease inhibitor I39 (alpha-2- macroglobulin) family. Protein type: Secreted; Inhibitor; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 12p13.31. Cellular Component: extracellular region; cytosol. Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; enzyme binding; protease binding; growth factor binding; interleukin-8 binding; interleukin-1 binding; calcium-dependent protein binding; tumor necrosis factor binding; receptor binding. Biological Process: negative regulation of complement activation, lectin pathway; platelet activation; extracellular matrix disassembly; extracellular matrix organization and biogenesis; regulation of small GTPase mediated signal transduction; platelet degranulation; small GTPase mediated signal transduction; stem cell differentiation; blood coagulation; blood coagulation, intrinsic pathway. Disease: Alzheimer Disease; Alpha-2-macroglobulin Deficiency

96 Tests

470 USD