protein

Recombinant Human TK1 Protein

MBS8249882

0.01 mg

255 USD

Recombinant Protein

Recombinant Human TK1 Protein

[TK1]

[Thymidine kinase, cytosolic]

[thymidine kinase, cytosolic isoform 1; Thymidine kinase, cytosolic; thymidine kinase, cytosolic; thymidine kinase 1]

[TK1]

[TK1; TK1; TK2]

KITH_HUMAN

Human cells

Human

234

MSCINLPTVL PGSPSKTRGQ IQVILGPMFS GKSTELMRRV RRFQIAQYKC LVIKYAKDTR YSSSFCTHDR NTMEALPACL LRDVAQEALG VAVIGIDEGQ FFPDIVEFCE AMANAGKTVI VAALDGTFQR KPFGAILNLV PLAESVVKLT AVCMECFREA AYTKRLGTEK EVEVIGGADK YHSVCRLCYF KKASGQPAGP DNKENCPVPG KPGEAVAARK LFAPQQILQC SPANVDHHHH HH

Greater than 95% as determined by reducing SDS-PAGE.

Liquid in a 0.2 uM filtered solution of PBS, pH 7.4.

Store it at -20°C to -80°C for one year.

ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)

SDS-PAGE

Endotoxin: Less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.

Directions for Use: Always centrifuge tubes before opening. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Recombinant Human TK1 Protein is produced by our mammalian expression system and the target gene encoding Met1-Asn234 is expressed with a 6His tag at the C-terminus.

164698438

NP_003249.3

NM_003258.4

P04183

Drug Metabolism - Other Enzymes Pathway (83033); Drug Metabolism - Other Enzymes Pathway (428); E2F Transcription Factor Network Pathway (137934); Fluoropyrimidine Activity Pathway (198778); Metabolic Pathways (132956); Metabolism Pathway (1269956); Metabolism Of Nucleotides Pathway (1270133); Pyrimidine Metabolism Pathway (1270138); Pyrimidine Metabolism Pathway (82946); Pyrimidine Metabolism Pathway (309)

The protein encoded by this gene is a cytosolic enzyme that catalyzes the addition of a gamma-phosphate group to thymidine. This creates dTMP and is the first step in the biosynthesis of dTTP, which is one component required for DNA replication. The encoded protein, whose levels fluctuate depending on the cell cycle stage, can act as a low activity dimer or a high activity tetramer. High levels of this protein have been used as a biomarker for diagnosing and categorizing many types of cancers. [provided by RefSeq, Oct 2016]

TK: cytosolic thymidine kinase. Phosphorylated during mitosis. Its enzymatic activity is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells. Protein type: EC 2.7.1.21; Kinase, other; Nucleotide Metabolism - pyrimidine; Xenobiotic Metabolism - drug metabolism - other enzymes. Chromosomal Location of Human Ortholog: 17q23.2-q25.3. Cellular Component: cytosol. Molecular Function: identical protein binding; nucleoside kinase activity; protein binding; zinc ion binding. Biological Process: DNA metabolic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; protein homotetramerization; pyrimidine nucleoside salvage; thymidine metabolic process

0.01 mg

255 USD

0.05 mg

635

0.5 mg

2370