Recombinant Human S100A12 Protein | MyBioSource MBS8249717 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human S100A12 Protein

catalog :

MBS8249717

quantity :

0.01 mg

price :

200 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS8249717

products type :

Recombinant Protein

products full name :

Recombinant Human S100A12 Protein

products short name :

[S100A12]

products name syn :

[Protein S100-A12; CGRP; Calcium-binding protein in amniotic fluid 1; CAAF1; Calgranulin-C; CAGC; Extracellular newly identified RAGE-binding protein; EN-RAGE; Migration inhibitory factor-related protein 6; MRP-6; p6; Neutrophil S100 protein; S100 calcium-binding protein A12]

other names :

[protein S100-A12; Protein S100-A12; protein S100-A12; S100 calcium binding protein A12; CGRP; Calcium-binding protein in amniotic fluid 1; CAAF1]

products gene name :

[S100A12]

other gene names :

[S100A12; S100A12; p6; CAGC; CGRP; MRP6; CAAF1; MRP-6; ENRAGE; CAAF1; CAGC; EN-RAGE; MRP-6; p6]

uniprot entry name :

S10AC_HUMAN

host :

E Coli

reactivity :

Human

sequence length :

sequence :

MTKLEEHLEG IVNIFHQYSV RKGHFDTLSK GELKQLLTKE LANTIKNIKD KAVIDEIFQG LDANQDEQVD FQEFISLVAI ALKAAHYHTH KE

purity :

Greater than 95% as determined by reducing SDS-PAGE.

form :

Lyophilized from a 0.2 uM filtered solution of PBS, pH 7.4.

storage stability :

Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.

tested application :

ELISA (EIA), Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)

other info1 :

Quality Control: Endotoxin: Less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.

other info2 :

Directions for Use: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

products description :

Recombinant Human S100A12 Protein is produced by our E Coli expression system and the target gene encoding Met1-Glu92 is expressed.

ncbi gi num :

5032059

ncbi acc num :

NP_005612.1

ncbi gb acc num :

NM_005621.1

uniprot acc num :

P80511

ncbi pathways :

Activated TLR4 Signalling Pathway (1269236); Advanced Glycosylation Endproduct Receptor Signaling Pathway (1269258); Cytosolic Sensors Of Pathogen-associated DNA Pathway (1269268); DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway (1269276); Immune System Pathway (1269170); Innate Immune System Pathway (1269203); MyD88 Cascade Initiated On Plasma Membrane Pathway (1269207); MyD88 Dependent Cascade Initiated On Endosome Pathway (1269228); MyD88-independent TLR3/TLR4 Cascade Pathway (1269220); MyD88:Mal Cascade Initiated On Plasma Membrane Pathway (1269237)

ncbi summary :

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein is proposed to be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities. The protein includes an antimicrobial peptide which has antibacterial activity. [provided by RefSeq, Nov 2014]

uniprot summary :

S100A12: S100A12 is a calcium-, zinc- and copper-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. Its proinflammatory activity involves recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to receptor for advanced glycation endproducts (AGER). Binding to AGER activates the MAP-kinase and NF-kappa-B signaling pathways leading to production of proinflammatory cytokines and up-regulation of cell adhesion molecules ICAM1 and VCAM1. Acts as a monocyte and mast cell chemoattractant. Can stimulate mast cell degranulation and activation which generates chemokines, histamine and cytokines inducing further leukocyte recruitment to the sites of inflammation. Can inhibit the activity of matrix metalloproteinases; MMP2, MMP3 and MMP9 by chelating Zn(2+) from their active sites. Possesses filariacidal and filariastatic activity. Calcitermin possesses antifungal activity against C.albicans and is also active against E.coli and P.aeruginosa but not L.monocytogenes and S.aureus. Belongs to the S-101 family. Chromosomal Location of Human Ortholog: 1q21. Cellular Component: cytoplasm; cytoskeleton; cytosol; extracellular region; nucleus; plasma membrane. Molecular Function: calcium ion binding; copper ion binding; protein binding; RAGE receptor binding; zinc ion binding. Biological Process: activation of NF-kappaB transcription factor; cytokine secretion; defense response to bacterium; defense response to fungus; innate immune response; mast cell activation; monocyte chemotaxis; neutrophil chemotaxis; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of inflammatory response; positive regulation of MAP kinase activity; xenobiotic metabolic process

size1 :

0.01 mg

price1 :

200 USD

size2 :

0.05 mg

price2 :

435

size3 :

0.5 mg

price3 :

2670

more info or order :

MyBioSource product webpage