ELISA/assay

Rat IL-1 beta ELISA Kit

MBS824956

96 Tests

470 USD

ELISA Kit

Rat IL-1 beta ELISA Kit

IL-1 beta

IL1F2; Interleukin-1 beta; IL-1 beta; Catabolin

Interleukin-1 beta; Interleukin-1 beta; interleukin-1 beta; IL-1 beta; interleukin 1 beta

IL1B

Il1b; Il1b; IL-1 beta

IL1B_RAT

Rat

268

The Rat IL-1 beta ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat IL-1 beta proteins within the range of 31.25 pg/ml - 2000 pg/ml.

Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Sandwich ELISA (SE)

Samples: Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates. Assay Type: Sandwich. Sensitivity: The minimum detectable dose of Rat IL-1 beta is typically less than 15 pg/ml.

Background/Introduction: Interleukin-1beta (IL-1beta) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB)-regulated genes. Furthermore, Microenvironmental IL-1beta and, to a lesser extent, IL-1alpha are required for in vivo angiogenesis and invasiveness of different tumor cells. Additional, the cooperation of IL-1beta and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture. Moreover, the association with disease may be explained by the biologic properties of IL-1beta, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion. Principle of the Assay: The Bioscience Rat IL-1 beta ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat IL-1 beta in Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates. This assay employs an antibody specific for Rat IL-1 beta coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat IL-1 beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

2497335

Q63264.1

Q63264

30,644 Da

African Trypanosomiasis Pathway (194377); African Trypanosomiasis Pathway (194323); Alzheimer's Disease Pathway (83489); Alzheimer's Disease Pathway (509); Amoebiasis Pathway (167311); Amoebiasis Pathway (167191); Apoptosis Pathway (83452); Apoptosis Pathway (470); Chagas Disease (American Trypanosomiasis) Pathway (147806); Chagas Disease (American Trypanosomiasis) Pathway (147795)

an inflammatory cytokine [RGD, Feb 2006]

IL1B: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. Monomer. Belongs to the IL-1 family. Protein type: Cytokine. Cellular Component: extracellular space; extracellular region; vesicle; secretory granule. Molecular Function: protein domain specific binding; interleukin-1 receptor binding; cytokine activity. Biological Process: positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of JNK activity; negative regulation of MAP kinase activity; positive regulation of nitric oxide biosynthetic process; glycoprotein metabolic process; negative regulation of glutamate secretion; positive regulation of apoptosis; activation of MAPK activity; positive regulation of interleukin-2 biosynthetic process; positive regulation of transcription, DNA-dependent; response to glucocorticoid stimulus; germ cell programmed cell death; negative regulation of insulin receptor signaling pathway; response to lipopolysaccharide; positive regulation of glial cell differentiation; positive regulation of NF-kappaB import into nucleus; positive regulation of lipid catabolic process; fever; response to organic cyclic substance; positive regulation of membrane protein ectodomain proteolysis; response to carbohydrate stimulus; activation of NF-kappaB transcription factor; elevation of cytosolic calcium ion concentration; response to vitamin D; pentacyclic triterpenoid metabolic process; positive regulation of phagocytosis; positive regulation of T cell proliferation; positive regulation of astrocyte differentiation; response to drug; neutrophil chemotaxis; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of heterotypic cell-cell adhesion; positive regulation of mitosis; interleukin-1 beta production; positive regulation of interleukin-6 production; social behavior; response to organic nitrogen; purine base metabolic process; negative regulation of neuron differentiation; positive regulation of angiogenesis; response to ethanol; response to heat; positive regulation of cell division; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; negative regulation of lipid metabolic process; leukocyte migration; estrogen metabolic process; response to peptide hormone stimulus; sequestering of triacylglycerol; wound healing; positive regulation of interleukin-6 biosynthetic process; response to morphine; positive regulation of JNK cascade; negative regulation of transcription from RNA polymerase II promoter; response to L-ascorbic acid; chronic inflammatory response to antigenic stimulus; response to estradiol stimulus; positive regulation of stress-activated MAPK cascade; negative regulation of neurogenesis; positive regulation of interleukin-8 production; negative regulation of cell proliferation; learning and/or memory; negative regulation of lipid catabolic process; hyaluronan biosynthetic process; protein kinase B signaling cascade; lipopolysaccharide-mediated signaling pathway; response to gamma radiation; inflammatory response; regulation of I-kappaB kinase/NF-kappaB cascade; response to nutrient; aging; cytokine and chemokine mediated signaling pathway; MAPKKK cascade; positive regulation of immature T cell proliferation in the thymus; response to ATP; memory; ovulation; polyketide metabolic process; positive regulation of interferon-gamma production; positive regulation of chemokine biosynthetic process; response to ozone; positive regulation of prostaglandin secretion; positive regulation of fever; response to hypoxia; immune response; positive regulation of protein amino acid phosphorylation; regulation of insulin secretion

96 Tests

470 USD