catalog number :
MBS8249356
products type :
Recombinant Protein
products full name :
Recombinant E Coli LACI Protein
products short name :
[LACI]
products name syn :
[Lactose operon repressor]
other names :
[lactose-inducible lac operon transcriptional repressor; Lactose operon repressor; lactose-inducible lac operon transcriptional repressor]
products gene name :
[LACI]
other gene names :
[lacI; lacI; ECK0342; JW0336]
uniprot entry name :
LACI_ECOLI
sequence :
MKPVTLYDVA EYAGVSYQTV SRVVNQASHV SAKTREKVEA AMAELNYIPN RVAQQLAGKQ SLLIGVATSS LALHAPSQIV AAIKSRADQL GASVVVSMVE RSGVEACKAA VHNLLAQRVS GLIINYPLDD QDAIAVEAAC TNVPALFLDV SDQTPINSII FSHEDGTRLG VEHLVALGHQ QIALLAGPLS SVSARLRLAG WHKYLTRNQI QPIAEREGDW SAMSGFQQTM QMLNEGIVPT AMLVANDQMA LGAMRAITES GLRVGADISV VGYDDTEDSS CYIPPLTTIK QDFRLLGQTS VDRLLQLSQG QAVKGNQLLP VSLVKRKTTL APNTQTASPR ALADSLMQLA RQVSRLESGQ HHHHHH
purity :
Greater than 95% as determined by reducing SDS-PAGE.
form :
Supplied as a 0.2 muM filtered solution of 20mM Tris, 300mM NaCl, 5mM DTT, pH 8.0.
storage stability :
Store it at -20°C to -80°C for one year.
tested application :
ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
other info1 :
Endotoxin: Less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test. Source: E Coli
other info2 :
Directions for Use: Always centrifuge tubes before opening. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
products description :
Recombinant E Coli LACI Protein is produced by our E Coli expression system and the target gene encoding Met1-Gln360 is expressed with a 6His tag at the C-terminus.
ncbi acc num :
NP_414879.3
ncbi gb acc num :
NC_000913.3
ncbi mol weight :
38,590 Da
ncbi summary :
The Lactose inhibitor," LacI, is a DNA-binding transcription factor that represses transcription of the operon involved in transport and catabolism of lactose . [More information is available at EcoCyc: EG10525].
uniprot summary :
Repressor of the lactose operon. Binds allolactose as an inducer.