Mouse Complement C5 ELISA Kit | MyBioSource MBS824906 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Complement C5 ELISA Kit

catalog :

MBS824906

quantity :

96 Tests

price :

490 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS824906

products type :

ELISA Kit

products full name :

Mouse Complement C5 ELISA Kit

products short name :

[Complement C5]

products name syn :

[CPAMD4; Complement C5; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4]

other names :

[complement C5 preproprotein; Complement C5; complement C5; hemolytic complement; Hemolytic complementCleaved into the following 4 chains:Complement C5 beta chain; Complement C5 alpha chain; C5a anaphylatoxin; Complement C5 alpha' chain]

products gene name :

[C5]

other gene names :

[Hc; C5; C5; He; C5a; Hc]

uniprot entry name :

CO5_MOUSE

reactivity :

Mouse

sequence length :

1680

specificity :

The Mouse CD25 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse CD25 proteins within the range of 78 pg/ml-5000 pg/ml.

storage stability :

Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

tested application :

Sandwich ELISA (SE)

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Cell Culture Supernatants, Serum, Plasma. Sensitivity: 5 pg/ml.

products description :

Principle of the Assay: The MyBioSource' Mouse CD25 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CD25 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse CD25 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD25 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CD25 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD25 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm!!Background/Introduction: CD25 is the alpha chain of the IL-2 receptor. It is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes that associates with CD122 to form a heterodimer that can act as a high-affinity receptor for IL-2. CD25 has been used as a marker to identify CD4+FoxP3+ regulatory T cells in mice. By in situ hybridization, the IL2R gene was mapped to 10p15-p14. Its soluble form, called sIL-2R may be elevated in these diseases and is occasionally used to track disease progression.

ncbi gi num :

6754164

ncbi acc num :

NP_034536.1

ncbi gb acc num :

NM_010406.2

uniprot acc num :

P06684

ncbi mol weight :

188,878 Da

ncbi pathways :

Activation Of C3 And C5 Pathway (1001244); Class A/1 (Rhodopsin-like Receptors) Pathway (1000942); Complement Activation, Classical Pathway (198379); Complement And Coagulation Cascades Pathway (198335); Complement And Coagulation Cascades Pathway (83270); Complement And Coagulation Cascades Pathway (484); Complement Cascade Pathway (1001237); G Alpha (i) Signalling Events Pathway (1000973); GPCR Downstream Signaling Pathway (1000971); GPCR Ligand Binding Pathway (1000941)

uniprot summary :

C5: Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. Defects in C5 are the cause of complement component 5 deficiency (C5D). A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. An association study of C5 haplotypes and genotypes in individuals with chronic hepatitis C virus infection shows that individuals homozygous for the C5_1 haplotype have a significantly higher stage of liver fibrosis than individuals carrying at least 1 other allele (PubMed:15995705). Protein type: Secreted, signal peptide; Secreted. Cellular Component: membrane attack complex; extracellular space; extracellular region. Molecular Function: endopeptidase inhibitor activity. Biological Process: positive regulation of angiogenesis; immune system process; in utero embryonic development; cytolysis; complement activation, alternative pathway; innate immune response; inflammatory response; complement activation, classical pathway

size1 :

96 Tests

price1 :

490 USD

more info or order :

MyBioSource product webpage