catalog number :
MBS8248744
products type :
Recombinant Protein
products full name :
Recombinant Human CD99 Protein
products short name :
[CD99]
products name syn :
[MIC2; MIC2X; MIC2Y; CD99 antigen; 12E7; E2 antigen; Protein MIC2; T-cell surface glycoprotein E2; CD99]
other names :
[CD99 antigen isoform b; CD99 antigen; CD99 antigen; CD99 molecule; 12E7; E2 antigen; Protein MIC2; T-cell surface glycoprotein E2; CD_antigen: CD99]
products gene name :
[CD99]
other gene names :
[CD99; CD99; MIC2; HBA71; MIC2X; MIC2Y; MSK5X; MIC2; MIC2X; MIC2Y]
uniprot entry name :
CD99_HUMAN
sequence :
DGGFDLSDAL PDNENKKPTA IPKKPSAGDD FDLGDAVVDG ENDDPRPPNP PKPMPNPNPN HPSSSGSFSD ADLADGVSGG EGKGGSDGGG SHRKEGEEAD VDHHHHHH
purity :
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
form :
Lyophilized from a 0.2 muM filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
storage stability :
Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.
tested application :
ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
other info1 :
Source: Human cells. Endotoxin: Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
other info2 :
Directions for Use: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
products description :
Recombinant Human CD99 Protein is produced by our Mammalian expression system and the target gene encoding Asp23-Asp122 is expressed with a 6His tag at the C-terminus.
ncbi acc num :
NP_001116370.1
ncbi gb acc num :
NM_001122898.2
ncbi mol weight :
17,128 Da
ncbi pathways :
Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cell Surface Interactions At The Vascular Wall Pathway (1269373); Hemostasis Pathway (1269340); Leukocyte Transendothelial Migration Pathway (83083); Leukocyte Transendothelial Migration Pathway (494)
ncbi summary :
The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. There is a related pseudogene located immediately adjacent to this locus. [provided by RefSeq, Mar 2016]
uniprot summary :
CD99: Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes. Belongs to the CD99 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: Xp22.32 and Yp11.3. Cellular Component: cytoplasm; focal adhesion; integral to plasma membrane; plasma membrane