ELISA/assay

Rat BDNF ELISA Kit

MBS824814

96 Tests

515 USD

ELISA Kit

Rat BDNF ELISA Kit

[BDNF]

[Brain-derived neurotrophic factor; BDNF; Abrineurin]

[brain-derived neurotrophic factor isoform 1; Brain-derived neurotrophic factor; brain-derived neurotrophic factor; brain derived neurotrophic factor; brain derived neurothrophic factor; brain-derived neurotrophic factor]

[BDNF]

[Bdnf; Bdnf; BDNF]

BDNF_RAT

Rat

362

The Mouse VEGF ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse VEGF proteins within the range of 15.6 pg/ml-1000 pg/ml.

Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Sandwich ELISA (SE)

Typical Testing Data/Standard Curve (for reference only)

Samples: Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates. Sensitivity: 7 pg/ml.

Principle of the Assay: The MyBioSource Mouse VEGF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse VEGF in Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates. This assay employs an antibody specific for Mouse VEGF coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse VEGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm!!Background/Introduction: Vascular permeability factor/vascular endothelial growth factor (VPF/VEGF), a potent cytokine expressed by most malignant tumors, has critical roles in vasculogenesis and both physiological and pathological angiogenesis. VEGF produced by tumor cells potently stimulates endothelial cell proliferation and angiogenesis and plays a key role in the pathophysiology of several neoplasias.VEGF may also play a pivotal role in mediating the development and progression of diabetic retinopathy.VEGF, a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk-1 and Flt-1. The VEGF gene is mapped by fluorescence in situ hybridization to chromosome 6p12.

397174764

NP_001257559.1

NM_001270630.1

P23363

28,109 Da

Alcoholism Pathway (585585); Alcoholism Pathway (587116); Brain Derived Neurotrophic Factor Pathway (755433); Cocaine Addiction Pathway (546271); Cocaine Addiction Pathway (546273); Huntington's Disease Pathway (83492); Huntington's Disease Pathway (512); MAPK Signaling Pathway (198496); MAPK Signaling Pathway (83440); MAPK Signaling Pathway (456)

plays a role in the development of hippocampal long term potentiation; involved in regulation of synaptic plasticity [RGD, Feb 2006]

During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS ().

96 Tests

515 USD