Anti-Alpha-1D Adrenergic Receptor Antibody | MyBioSource MBS8248057 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Alpha-1D Adrenergic Receptor Antibody

catalog :

MBS8248057

quantity :

0.03 mL

price :

155 USD

clonality :

polyclonal

host :

unidentified

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of Alpha-1D Adrenergic Receptor expression in MCF7 (A), RAW264.7 (B), H9C2 (C) whole cell lysates.

image 2 :

Immunohistochemical analysis of Alpha-1D Adrenergic Receptor staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

image 3 :

Immunofluorescent analysis of Alpha-1D Adrenergic Receptor staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

product information

catalog number :

MBS8248057

products type :

Antibody

products full name :

Anti-Alpha-1D Adrenergic Receptor Antibody

products short name :

[Alpha-1D Adrenergic Receptor]

products name syn :

[ADRA1A; Alpha-1D adrenergic receptor; Alpha-1A adrenergic receptor; Alpha-1D adrenoreceptor; Alpha-1D adrenoceptor; Alpha-adrenergic receptor 1a]

other names :

[alpha-1D adrenergic receptor; Alpha-1D adrenergic receptor; alpha-1D adrenergic receptor; adrenoceptor alpha 1D; Alpha-1A adrenergic receptor; Alpha-1D adrenoreceptor; Alpha-1D adrenoceptor; Alpha-adrenergic receptor 1a]

products gene name :

[ADRA1D]

other gene names :

[ADRA1D; ADRA1D; DAR; ADRA1; ADRA1A; ADRA1R; ALPHA1; dJ779E11.2; ADRA1A; Alpha-1D adrenoceptor]

uniprot entry name :

ADA1D_HUMAN

clonality :

Polyclonal

host :

unknown

reactivity :

Human, Mouse, Rat

sequence length :

572

specificity :

Recognizes endogenous levels of Alpha-1D Adrenergic Receptor protein.

purity :

The antibody was purified by affinity chromatography.

form :

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

storage stability :

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

app notes :

WB: 1/500 - 1/2000. IHC: 1/50 - 1/200. IF/ICC: 1/50 - 1/100

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunofluorescence (IF)

other info1 :

Source: Rabbit. Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Alpha-1D Adrenergic Receptor. The exact sequence is proprietary.

products description :

Rabbit polyclonal antibody to Alpha-1D Adrenergic Receptor

ncbi gi num :

4501957

ncbi acc num :

NP_000669.1

ncbi gb acc num :

NM_000678.3

uniprot acc num :

P25100

ncbi mol weight :

60,463 Da

ncbi pathways :

Adrenergic Signaling In Cardiomyocytes Pathway (908257); Adrenergic Signaling In Cardiomyocytes Pathway (909696); Adrenoceptors Pathway (1269555); Amine Ligand-binding Receptors Pathway (1269553); Calcium Regulation In The Cardiac Cell Pathway (198906); Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Class A/1 (Rhodopsin-like Receptors) Pathway (1269545); G Alpha (12/13) Signalling Events Pathway (1269579); G Alpha (q) Signalling Events Pathway (1269578)

ncbi summary :

Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. They activate mitogenic responses and regulate growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. This gene encodes alpha-1D-adrenergic receptor. Similar to alpha-1B-adrenergic receptor gene, this gene comprises 2 exons and a single intron that interrupts the coding region. [provided by RefSeq, Jul 2008]

uniprot summary :

ADRA1D: This alpha-adrenergic receptor mediates its effect through the influx of extracellular calcium. Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA1D sub-subfamily. Protein type: GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR. Chromosomal Location of Human Ortholog: 20p13. Cellular Component: integral to plasma membrane; plasma membrane. Molecular Function: protein binding. Biological Process: cell proliferation; cell-cell signaling; DNA metabolic process; elevation of cytosolic calcium ion concentration; G-protein coupled receptor protein signaling pathway; G-protein signaling, coupled to cAMP nucleotide second messenger; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); multicellular organismal development; norepinephrine-epinephrine vasoconstriction involved in regulation of systemic arterial blood pressure; positive regulation of cell proliferation; positive regulation of smooth muscle contraction; positive regulation of vasoconstriction

size1 :

0.03 mL

price1 :

155 USD

size2 :

0.1 mL

price2 :

220

size3 :

0.2 mL

price3 :

295

more info or order :

MyBioSource product webpage