Rat Fibronectin ELISA Kit | MyBioSource MBS824794 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Fibronectin ELISA Kit

catalog :

MBS824794

quantity :

96 Tests

price :

515 USD

product information

catalog number :

MBS824794

products type :

ELISA Kit

products full name :

Rat Fibronectin ELISA Kit

products short name :

[Fibronectin]

products name syn :

[FN; Fibronectin; FN; Cold-insoluble globulin; CIG]

other names :

[Fibronectin; Fibronectin; fibronectin; FN; fibronectin 1]

products gene name :

[FN1]

other gene names :

[Fn1; Fn1; fn-1; FIBNEC; FN]

uniprot entry name :

FINC_RAT

reactivity :

Rat

sequence length :

2477

specificity :

The Rat Fibronectin ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat Fibronectin proteins within the range of 156 pg/ml - 10000 pg/ml.

storage stability :

Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

tested application :

Sandwich ELISA (SE)

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Cell Culture Supernatants, Serum, Plasma. Sensitivity: <15 pg/ml

products description :

Principle of the Assay: The Rat Fibronectin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat Fibronectin in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Rat Fibronectin coated on a 96-well plate. Standards and samples are pipetted into the wells and Fibronectin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat Fibronectin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Fibronectin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background: Fibronectin (FN) also known as LETS, is identified on the surfFN of fibroblasts by labeling with radioactive compounds or specific antibodies. Fibronectin is a 430,000-dalton dimeric glycoprotein that exists in 2 forms, termed cellular and plasma fibronectin. Cellular and plasma fibronectins are heterodimers consisting of similar but not identical polypeptides. These two forms of FN differ in biologic activity. Fibronectins bind cell surfFNs and various compounds including collagen, fibrin, heparin, DNA, and actin. Because fibronectin stimulates endocytosis in several systems and promotes the clearance of particulate material from the circulation, it could function in the clearance of C1q-coated material such as immune complexes or cellular debris. Fibronectins are involved in cell adhesion, cell motility, opsonization, would healing, and maintenance of cell shape. LETS, encoded on chromosome 8, is responsible for the LETS protein expression in humans. Because LETS has been implicated in tumorigenicity and cellular transformation, it is of interest that rearrangement or modifications in the number of chromosome 8 have been associated with certain forms of cancer.

ncbi gi num :

120178

ncbi acc num :

P04937.2

uniprot acc num :

P04937

ncbi mol weight :

259,350 Da

ncbi pathways :

Amoebiasis Pathway (167311); Amoebiasis Pathway (167191); Bacterial Invasion Of Epithelial Cells Pathway (149774); Bacterial Invasion Of Epithelial Cells Pathway (148661); Cell Surface Interactions At The Vascular Wall Pathway (1010919); ECM-receptor Interaction Pathway (83460); ECM-receptor Interaction Pathway (479); Extracellular Matrix Organization Pathway (1009923); Fibronectin Matrix Formation Pathway (1009929); Focal Adhesion Pathway (83459)

ncbi summary :

extracellular matrix component; may play a role in fibrosis and tumor metastasis [RGD, Feb 2006]

uniprot summary :

FN1: Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Defects in FN1 are the cause of glomerulopathy with fibronectin deposits type 2 (GFND2); also known as familial glomerular nephritis with fibronectin deposits or fibronectin glomerulopathy. GFND is a genetically heterogeneous autosomal dominant disorder characterized clinically by proteinuria, microscopic hematuria, and hypertension that leads to end-stage renal failure in the second to fifth decade of life. 15 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis; Secreted; Cell adhesion; Secreted, signal peptide. Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; apical plasma membrane; fibrinogen complex; ER-Golgi intermediate compartment; extracellular region; basement membrane; basal lamina. Molecular Function: integrin binding; heparin binding; protein binding; protease activator activity; protease binding; extracellular matrix structural constituent; mercury ion binding. Biological Process: integrin activation; extracellular matrix organization and biogenesis; ossification; wound healing; positive regulation of chemotaxis; positive regulation of axon extension; cell-matrix adhesion; response to glucocorticoid stimulus; glial cell migration; cell activation; regulation of cell shape; response to ozone; cell-substrate junction assembly; acute-phase response; calcium-independent cell-matrix adhesion; response to wounding; peptide cross-linking; angiogenesis; cell adhesion; negative regulation of apoptosis; positive regulation of cell migration

size1 :

96 Tests

price1 :

515 USD