ELISA/assay

Human COMP ELISA Kit

MBS824705

96 Tests

470 USD

ELISA Kit

Human COMP ELISA Kit

COMP

Cartilage oligomeric matrix protein; COMP; Thrombospondin-5; TSP5

cartilage oligomeric matrix protein; Cartilage oligomeric matrix protein; cartilage oligomeric matrix protein; TSP5; thrombospondin-5; pseudoachondroplasia (epiphyseal dysplasia 1, multiple); cartilage oligomeric matrix protein(pseudoachondroplasia, epiphyseal dysplasia 1, multiple); cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia 1, multiple); cartilage oligomeric matrix protein; Thrombospondin-5; TSP5

COMP

COMP; COMP; MED; EDM1; EPD1; PSACH; THBS5; COMP; TSP5

COMP_HUMAN

Human

757

The Human COMP ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human COMP proteins within the range of 156 pg/ml - 10000 pg/ml.

Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Sandwich ELISA (SE)

Samples: Sandwich Enzyme-Linked Immunosorbent Assay for Quantitative Detection of Human COMP Concentrations in Cell Culture Supernatants, Serum, Plasma. Sensitivity: The minimum detectable dose of Human COMP is typically less than 10 pg/ml.

Background/Introduction: Cartilage oligomeric matrix protein is a protein that in humans is encoded by the COMP gene. The sequences of rat and bovine COMP indicate that it is a member of the thrombospondin gene family. By Southern blot analysis of a somatic cell hybrid DNA panel and by isotopic in situ hybridization, human COMP gene was mapped to 19p13.1, and the murine COMP gene was mapped to the central region of mouse chromosome 8 by use of an interspecific backcross mapping panel. COMP is a marker of cartilage turnover. Principle of the Assay: The Bioscience Human COMP ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human COMP in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human COMP coated on a 96-well plate. Standards and samples are pipetted into the wells and COMP present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human COMP antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of COMP bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

40217843

NP_000086.2

NM_000095.2

P49747

77,214 Da

ECM Proteoglycans Pathway (833812); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Extracellular Matrix Organization Pathway (576262); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); Integrin Cell Surface Interactions Pathway (106110); Malaria Pathway (152665); Malaria Pathway (152657)

The protein encoded by this gene is a noncollagenous extracellular matrix (ECM) protein. It consists of five identical glycoprotein subunits, each with EGF-like and calcium-binding (thrombospondin-like) domains. Oligomerization results from formation of a five-stranded coiled coil and disulfides. Binding to other ECM proteins such as collagen appears to depend on divalent cations. Mutations can cause the osteochondrodysplasias pseudochondroplasia (PSACH) and multiple epiphyseal dysplasia (MED). [provided by RefSeq, Jul 2008]

COMP: May play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Could play a role in the pathogenesis of osteoarthritis. Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7. Defects in COMP are the cause of multiple epiphyseal dysplasia type 1 (EDM1). EDM is a generalized skeletal dysplasia associated with significant morbidity. Joint pain, joint deformity, waddling gait, and short stature are the main clinical signs and symptoms. EDM is broadly categorized into the more severe Fairbank and the milder Ribbing types. Defects in COMP are the cause of pseudoachondroplasia (PSACH). PSAC is a dominantly inherited chondrodysplasia characterized by short stature and early-onset osteoarthrosis. PSACH is more severe than EDM1 and is recognized in early childhood. Belongs to the thrombospondin family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 19p13.1. Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; extracellular region. Molecular Function: heparin binding; heparan sulfate proteoglycan binding; collagen binding; protein binding; protease binding; extracellular matrix structural constituent; calcium ion binding. Biological Process: limb development; organ morphogenesis; extracellular matrix organization and biogenesis; apoptosis; cell adhesion; skeletal development; negative regulation of apoptosis. Disease: Pseudoachondroplasia; Epiphyseal Dysplasia, Multiple, 1

96 Tests

470 USD