ELISA/assay

Human Periostin ELISA Kit

MBS824679

96 Tests

490 USD

ELISA Kit

Human Periostin ELISA Kit

[Periostin]

[OSF2; Periostin; PN; Osteoblast-specific factor 2; OSF-2]

[periostin isoform 2; Periostin; periostin; periodontal ligament-specific periostin; osteoblast specific factor 2 (fasciclin I-like); periostin, osteoblast specific factor; Osteoblast-specific factor 2; OSF-2]

[POSTN]

[POSTN; POSTN; PN; OSF2; OSF-2; PDLPOSTN; periostin; OSF2; PN; OSF-2]

POSTN_HUMAN

Human

779

The Human MERTK ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human MERTK proteins within the range of 156 pg/ml-10000 pg/ml.

Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Sandwich ELISA (SE)

Typical Testing Data/Standard Curve (for reference only)

Samples: Cell Culture Supernatants, Cell Lysates, Tissue Homogenates, Serum. Sensitivity: 10 pg/ml.

Principle of the Assay: The Human MERTK ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MERTK in Cell Culture Supernatants, Cell Lysates, Tissue Homogenates, Serum. This assay employs an antibody specific for Human MERTK coated on a 96-well plate. Standards and samples are pipetted into the wells and MERTK present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MERTK antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MERTK bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm!!Background/Introduction: Proto-oncogene tyrosine-protein kinase MER is an enzyme that in humans is encoded by the MERTK gene. This gene is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type (immunoglobulin-like) domains, and one tyrosine kinase domain. Its gene is mapped to chromosome 2q14.1. Mer encodes a 984-amino acid protein with a calculated molecular mass of 109 kD. It is expressed in numerous neoplastic B-and T-cell lines. Mutations in this gene have been associated with disruption of the retinal pigment epithelium (RPE) phagocytosis pathway and onset of autosomal recessive retinitis pigmentosa (RP)

209862911

NP_001129406.1

NM_001135934.1

Q15063

80,357 Da

This gene encodes a secreted extracellular matrix protein that functions in tissue development and regeneration, including wound healing, and ventricular remodeling following myocardial infarction. The encoded protein binds to integrins to support adhesion and migration of epithelial cells. This protein plays a role in cancer stem cell maintenance and metastasis. Mice lacking this gene exhibit cardiac valve disease, and skeletal and dental defects. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]

POSTN: Binds to heparin. Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Cell adhesion; Secreted, signal peptide; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 13q13.3. Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; trans-Golgi network. Molecular Function: heparin binding; protein binding; cell adhesion molecule binding. Biological Process: tissue development; extracellular matrix organization and biogenesis; regulation of Notch signaling pathway; cell adhesion; skeletal development

96 Tests

490 USD